超高效液相色谱-串联质谱法同时测定保健酒中14种黄酮类成分的含量

Simultaneous determination of 14 kinds of flavones in health-care wine by ultra performance liquid chromatography-tandem mass spectrometry

目的建立超高效液相色谱-串联质谱法同时测定保健酒中14种黄酮类成分含量的方法。方法样品经乙腈水(2:8,V:V)稀释10倍后,采用Luna■C8(2)100A(150 mm×2 mm,3μm)柱以0.1%甲酸溶液和乙腈为流动相进行梯度洗脱分离,电喷雾负离子化(electron spray ionization,ESI-)和多反应监测模式(multiple reaction monitoring,MRM)检测。结果在0~20.0μg/L范围内,14种目标化合物呈良好的线性关系,相关系数(r^2)大于0.99,方法的定量限(S/N=10)为0.4?33.0μg/L;14种黄酮类化合物在10.0、50.0和100.0μg/L 3个水平下的平均加标回收率为78.8%~96.7%,相对标准偏差(RSD,n=6)为2.8%~15.1%。结论该方法简单、高效、灵敏、准确,可用于同时测定保健酒中14种黄酮类成分的含量。

Objective To establish a method for simultaneous determination of 14 kinds of flavones in health-care wine by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods The sample was diluted with acetonitrile-water(2:8,V:V)for 10 times.The separation was performed on a Luna■C8(2)column(100 A,150 mm×2 mm,3μm)using a mobile phase gradient with 0.1%formic acid and acetonitrile.The determination was carried out with electrospray ionsource under the negative mode with multiple-reaction monitoring(MRM)mode.Results This method had good linear relationships with correlation coefficients(r^2)not less than 0.99 in the range of 020.0μg/L.The limits of quantification(LOQ,S/N=10)of 14 kinds of flavones were varied from 0.4μg/L to 33.0μg/L.At the spiked levels of 10.0,50.0 and 100.0μg/L,the average recoveries of the 14 kinds of flavones ranged from 78.8%to 96.7%,and the relative standard deviation(RSD,n=6)was 2.8%15.1%.Conclusion This method is simple,efficient,sensitive,and accurate,and can be used to detect the 14 kinds of flavones in health-care wine.