高效液相色谱-串联质谱法测定茶叶中47种农药残留

Determination of 47 kinds of pesticide residues in tea by high performance liquid chromatography-tandem mass spectrometry

目的建立一种高效液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)测定茶叶中47种农药残留的分析方法。方法样品经乙腈、低温超声提取,采用改进的QuEChERS方法对样品进行净化处理,有效降低样品中复杂基质所带来的背景干扰,再经LC-MS/MS分析,外标定量。结果47种农残在1~50μg/L范围内具有较好的线性关系,相关系数r均大于0.995。加标水平为5、10、20μg/kg时,47种农药的回收率在60%~120%,相对标准偏差小于10%,同时对3种不同的茶叶进行加标回收率分析,回收率在60%~120%。结论该方法快速、准确、灵敏,适合测定适用于茶叶中47种农药残留的检测。

Objective To establish a sensitive method for the determination of 47 kinds of pesticide residues in tea by liquid chromatography-tandem mass spectrometry(LC-MS/MS).Methods The samples were extracted with acetonitrile,followed by ultrasonic extraction at lower temperatures.The modified QuEChERS method was used to purify extraction solution for removing interference matrix from agricultural products,purified samples was analysed by LC-MS/MS and quantified by the external standard method.Results The calibration curves of 47 kinds of pesticide residues were linear in the range of 1-50μg/L with correlation coefficients more than 0.995.The average recoveries of the 47 kinds of pesticides were almost in the range of 60%-120%at the spiked levels of 5,10,20μg/kg,and the relative standard deviations were less than 10%.At the same time,the recovery rates of three kinds of different tea were 60%-120%.Conclusion This method is rapid,accurate and sensitive,and could meet the requirement for the determination of the 47 kinds of pesticide residues in tea.