甘薯提取物对结直肠癌荷瘤鼠粪便炎症相关指标的影响

Effects of sweet potato extract on the inflammation-related fecal indexes in mice transplanted with colorectal cancer

目的探索甘薯提取物对结直肠癌荷瘤裸鼠粪便中钙卫蛋白(calprotectin,CP)、乳铁蛋白(lactoferrin,LTF)、二胺氧化酶(diamine oxidase,DAO)及氨基酸含量的影响,分析其可能的作用机理。方法将12只雄性裸鼠随机分为4组:空白对照组(CG1)、甘薯提取物组(CG2)、移植瘤模型组(TCG)、移植瘤模型+甘薯提取物组(TTG)。每组3只小鼠,分别干预1个月,在第23、30 d收集粪便检测CP、LTF、DAO和氨基酸含量。结果实验第23 d TCG组CP、LTF和DAO含量即均显著高于CG1组(P<0.05),TTG组LTF和DAO含量比TCG组显著降低(P<0.05);到第30 d时,TTG组CP和LTF含量显著低于TCG组(P<0.05),但DAO显著升高(P<0.05)。此外,CG2组动物粪便中16种氨基酸含量比CG1组均升高(P<0.05);TTG组除缬氨酸、蛋氨酸、异亮氨酸、酪氨酸和组氨酸外其余氨基酸含量比TCG组均升高(P<0.05)。结论经口摄入甘薯提取物有助于改善荷瘤鼠体内的氨基酸代谢失衡状况,显著降低肠道炎症介质CP和LTF的水平,并提高DAO含量,有助于保护肠粘膜的完整性和抑制肿瘤。

Objective To explore the effect of sweet potato extract on calprotectin(CP),lactoferrin(LTF),diamine oxidase(DAO)and amino acid content in the stool of colorectal cancer-bearing nude mice,and analyze its possible mechanism.Methods Twelve male nude mice were randomly divided into four groups:blank control group(CG1),sweet potato extract group(CG2),transplanted tumor model group(TCG),transplanted tumor model+sweet potato extract group(TTG).Three mice in each group were intervened for one month,and feces were collected on days 23 and 30 to detect CP,LTF,DAO and amino acid content.Results The contents of CP,LTF and DAO in the TCG group on the 23rd day of the experiment were significantly higher than those in the CG1 group(P<0.05),and the contents of LTF and DAO in the TTG group were significantly lower than those in the TCG group(P<0.05);on the 30th day,the contents of CP and LTF in TTG group were significantly lower than those in TCG group(P<0.05),but DAO was significantly increased(P<0.05).In addition,the content of 16 amino acids in animal feces of the CG2 group was higher than that of the CG1 group(P<0.05);the amino acid content of the TTG group except valine,methionine,isoleucine,tyrosine and histidine was higher than that of TCG groups(P<0.05).Conclusion Oral intake of sweet potato extract can improve the imbalance of amino acid metabolism in tumor-bearing mice,significantly reduce the levels of intestinal inflammatory mediators CP and LTF,and increase the content of DAO,which can help to protect the integrity of intestinal mucosa and inhibit tumor.