聊城市市售豆芽菜中5种植物生长调节剂残留调查分析

Investigation and analysis of 5 plant growth regulator residues in commercial sprouts in Liaocheng city

目的分析聊城市东昌府区市售绿豆芽和黄豆芽中6-苄基腺嘌呤、4-氯苯氧乙酸、2,4-二氯苯氧乙酸、吲哚乙酸、吲哚丁酸的残留情况。方法样品采用QuEChERs方法提取,用含有0.1%甲酸的5 mmol/L乙酸铵水(A)和甲醇(B)作为流动相进行梯度洗脱,质谱采用多反应监测-信息依赖性采集-增强子离子扫描模式(multiple reaction monitoring-information dependent acquisition-enhanced product ion,MRM-IDA-EPI),同时得到的子离子谱图用于谱图库检索进行定性确证分析。结果绿豆芽和黄豆芽中均存在植物生长调节剂残留,在所检测的20份样品中,10份检出6-苄基腺嘌呤,11份检出4-氯苯氧乙酸,20份检出吲哚乙酸,其他两项均未检出。阳性样品的谱图匹配度均高于70%。结论市售豆芽中植物生长调节剂的滥用依然存在,必须持续监测和控制豆芽中植物生长调节剂的残留,谨防滥用植物生长调节剂给消费者健康带来的风险。

Objective To analyze the residues of 6-benzylaminopurine(6-BAP),4-chlorophenoxyacetic acid(4-CPA),2,4-dichlorophenoxyacetic acid(2,4-D),indole-3-acetic acid(IAA)and 3-indolebutyric acid(IBA)in soybean sprouts and mung bean sprouts in Dongchangfu district,Liaocheng city.Methods The samples were extracted by QuEChERs method,and 5 mmol/L ammonium acetate water(A)and methanol(B)containing 0.1%formic acid were used as mobile phase for gradient elution.Multiple reaction monitoring-information dependent acquisition-enhanced product ion(MRM-IDA-EPI)was used for mass spectrometry,and the resulting sub-ion spectra were used to spectral database retrieval for qualitative confirmation analysis.Results Plant growth regulators were found in mung bean sprouts and soybean sprouts.Among the 20 samples tested,6-benzyl adenine was detected in 10,4-chlorophenoxyacetic acid in 11,and indoleacetic acid in 20,the other two items were not detected.The spectral matching degree of positive samples was more than 70%.Conclusion The abuse of plant growth regulators in commercially available bean sprouts still exists.It is necessary to continuously monitor and control the residues of plant growth regulators in bean sprouts,and to prevent the risks to consumers'health caused by the abuse of plant growth regulators.