基于DNA适配体的荧光生物传感器

DNA Aptamer-based Fluorescence Biosensor

近年来,随着DNA纳米技术的飞速发展,基于DNA作为适配体的荧光生物传感器不断被大量学者研究和构建,以实现对靶标物质的灵敏快速检测。作为DNA纳米技术的新兴方向,基于DNA适配体的荧光生物传感器具有巨大的应用潜力。该文对近年来基于DNA适配体所构建的荧光生物传感器进行了总结。包括荧光信号的实现:荧光染料标记;非荧光染料标记。荧光检测信号的提升:酶介导的靶标循环和信号扩增策略;链置换反应介导的靶标循环和信号扩增策略;基于链置换反应和酶介导的靶标循环和信号扩增策略。在此基础上对基于DNA适配体的荧光生物传感器进行展望并提出建议。

In recent years,with the rapid development of DNA nanotechnology,fluorescence biosensors based on DNA as aptamer are studied and constructed by a large number of scholars in order to realize the sensitive and rapid detection of target materials.As a new branch of DNA nanotechnology,fluorescence biosensors based on DNA aptamer have great application.The fluorescence biosensors based on DNA aptamers are summarized.The realization of fluorescence signal contains fluorescent dyes and non-fluorescent dye labeled.Enhancement of fluorescence signals involve enzyme-assisted,chain replacement reaction and both of all mediated target circulation and signals amplification strategy.On this basis,the fluorescence biosensor based on DNA aptamer is prospected and some suggestions are put forward.