基于体外细胞模型的太子参安全性与抗氧化活性系统评价

Systematic Evaluation of Safety and Antioxidant Activity of Pseudostellaria heterophylla Based on in Vitro Cell Model

为科学认识和系统评价太子参提取物在细胞水平上的安全性与抗氧化活性,根据PICOS检索策略检索国内外数据库,采用Stata软件进行Meta循证统计分析,共纳入11篇文献18项研究,总样本量为482。Meta分析显示:太子参试验组体外细胞毒性试验结果较对照组差异不显著[SMD=0.22,95%CI(-0.22,0.66),P=0.219>0.05];太子参提取物可显著提高体外试验细胞存活率[SMD=0.96,95%CI(0.15,1.77),P<0.05]和极显著降低细胞凋亡率[SMD=-2.38,95%CI(-3.21,-1.54),P<0.01],并且极显著提升胞内超氧化物歧化酶(SOD)活力[SMD=1.65,95%CI(0.97,2.32),P<0.01]和极显著降低胞内丙二醛(MDA)含量[SMD=-1.99,95%CI(-2.68,-1.29),P<0.01];对抑制体外红细胞溶血的系统描述结果为阳性,敏感分析结果稳健,发表偏倚弱。基于现有文献显示:太子参提取物在细胞水平上具有良好的安全性和抗氧化活性。

In order to scientifically understand and systematically evaluate the safety and antioxidant activity of Pseudostellaria heterophylla extract at the cellular level,the associated published literatures indexed in domestic and foreign databases were retrieved according to the PICOS search strategy,and Stata software was used for Meta evidence-based statistical analysis.A total of 18 studies in 11 articles were included,and the total samples were 482.Meta-analysis demonstrated that the Pseudostellaria heterophylla test group had no significant difference in in vitro cytotoxicity tests compared with control group[SMD=0.22,95%CI(-0.22,0.66),P=0.219>0.05];Pseudostellaria heterophylla significantly improved the cell survival rate[SMD=0.96,95%CI(0.15,1.77),P<0.05],significantly decreased the apoptosis rate[SMD=-2.38,95%CI(-3.21,-1.54),P<0.01],significantly elevated the intracellular superoxide dismutase(SOD)activity[SMD=1.65,95%CI(0.97,2.32),P<0.01],and significantly reduced the intracellular malondialdehyde(MDA)content[SMD=-1.99,95%CI(-2.68,-1.29),P<0.01]in the in vitro tests;the systematic description result of inhibiting hemolysis of erythrocytes in vitro was positive,the sensitivity analysis result was robust,and the publication bias was weak.Based on the existing literatures,Pseudostellaria heterophylla extract has good safety and antioxidant activity at the cellular level.