苎麻体细胞植株再生体系优化研究

Plant Regeneration System Optimization of Ramie Somatic Cells

旨在探索苎麻体细胞植株再生体系的优化方案,为苎麻组织培养及品种改良提供理论与技术依据。本试验以‘湘苎3号’为试材,优化苎麻愈伤诱导植株再生体系,再将此优化的再生体系应用于其他4个种质材料,得出5个种质的愈伤诱导率、芽诱导率、再生苗生根率,来比较不同基因型之间的体细胞植株再生率差异。结果表明‘,湘苎3号’最佳愈伤诱导培养基为附加2,4-D (0.1 mg/L)和6-BA (2.5 mg/L)的MS培养基;‘湘苎3号’、‘咸丰大叶绿’、‘A’、‘B’、‘中苎1号’的愈伤诱导率分别是:83.3%、80.0%、76.7%、70.0%、53.3%。‘湘苎3号’最佳芽诱导培养基为附加TDZ (0.5 mg/L)、2,4-D (0.02 mg/L)和IAA (0.03 mg/L)的1/2MS培养基,‘湘苎3号’、‘咸丰大叶绿’、‘A’、‘B’、‘中苎1号’的芽诱导率分别是:28.4%、21.2%、12.8%、8.9%、4.9%。‘湘苎3号’最佳生根培养基为附加TDZ (0.02 mg/L)+NAA(0.05 mg/L)+IAA (0.02 mg/L)的MS培养基,‘湘苎3号’、‘咸丰大叶绿’、‘A’、‘B’、‘中苎1号’的生根率都是100%。

This experiment aims at exploring the optimization scheme of plant regeneration system of ramie somatic cell and provide theoretical and technical basis for tissue culture and variety improvement of ramie. In this experiment,‘Xiangzhu 3’was used as the test material to optimize the plant regeneration system of ramie callus induction, and then the optimized regeneration system was applied to the other four germplasms of materials, and the callus induction rate, the bud induction rate and the regenerated seedling rooting rate of the five germplasms were obtained. Differences of plant regeneration rates of somatic cells were compared among different genotypes. The results showed that the optimum callus induction medium for‘Xiangzhu 3’was MS medium supplemented with 2,4-D(0.1 mg/L) and 6-BA(2.5 mg/L);the callus induction rate of‘Xiangzhu 3’,‘Xianfeng Dayelv’,‘A’,‘B’and‘Zhongzhu 1’was 83.3%, 80.0%, 76.7%, 70.0% and 53.3%, respectively.The optimum bud induction medium for‘Xiangzhu 3’was 1/2 MS medium supplemented with TDZ(0.5 mg/L),2,4-D(0.02 mg/L) and IAA(0.03 mg/L);the bud induction rate of‘Xiangzhu 3’,‘Xianfeng Dayelv’,‘A’,‘B’and‘Zhongzhu 1’was 28.4%, 21.2%, 12.8%, 8.9% and 4.9%, respectively. The optimum rooting medium of‘Xiangzhu 3’was MS medium supplemented with TDZ(0.02 mg/L) + NAA(0.05 mg/L) + IAA(0.02 mg/L).The rooting rate of‘Xiangzhu 3’,‘Xianfeng Dayelv’,‘A’,‘B’and‘Zhongzhu 1’were all 100%.