摘要
为了解在育种过程中各品系材料的品质状况,采用SDS-PAGE技术对选取的49份品系材料高分子量麦谷蛋白亚基的组成进行分析。结果表明:共发现8种等位变异和11种亚基组合类型。其中,GluA1位点出现2种亚基类型,以亚基1(83.67%)为主。Glu-B1位点出现4种亚基类型,以亚基14+15(42.86%)为主。Glu-D1位点出现2种亚基类型,亚基2+12(71.43%)和亚基5+10(28.57%)。对49份材料进行品质性状测定,并与各个亚基间做相关性分析。Glu-A1位点,亚基1的各个指标均高于亚基Null的指标。Glu-D1位点,亚基5+10的品质指标也高于亚基2+12。Glu-B1位点4种亚基在各个品质指标间均存在显著差异,亚基7+8对蛋白质含量、容重以及湿面筋的贡献最高,亚基17+18对沉淀值和最大抗延阻力的贡献最高,亚基7+9具有最长的稳定时间。
To study the quality of wheat strains during breeding process,we analyzed the HMW-GS composition of 49 wheat strains by using SDS-PAGE technique.The results showed that:8 HMW-GS alleles and 11 subunits combination were detected;2 types of subunits were on locus Glu-A1,subunit 1(83.67%)was the major subunit;4 types of subunits were on locus Glu-B1,subunit 14+15(42.86%)was the major subunit;subunit 2+12(71.43%)and 5+10(28.57%)were detected on locus Glu-D1;we determined the quality of the49 wheat strains and conducted the correlation analysis between the quality and the subunits;the determination result of subunit 1 on locus Glu-A1 was higher than that of subunit Null;on locus Glu-D1,the quality of subunit 5+10 was higher than that of subunit 2+12;on locus Glu-B1,there were significant differences between the 4 types of subunits in qualities;subunit 7+8 had the highest contribution to the protein content,bulk density and wet gluten content;subunit 17+18 had the highest contribution to Zeleny sedimentation and maximum anti-extension resistance;subunit 7+9 had the longest stability time.
作者
王倩
任文斌
邱玉亮
许琦
谢三刚
Wang Qian;Ren Wenbin;Qiu Yuliang;Xu Qi;Xie Sangang(Cotton Research Institute,Shanxi Academy of Agricultural Sciences,Yuncheng Shanxi 044000)
出处
《中国农学通报》
2020年第14期11-15,共5页
Chinese Agricultural Science Bulletin
基金
国家重点研发计划“小麦杂种优势利用技术与强优势杂交种创制”(2016YFD0101600)
山西省农科院优势课题组自选项目“晋南麦区高产优质小麦新品种选育”(YYS1709)
国家重点研发计划“黄淮冬麦区北片高产优质节水小麦新品种培育”(2017YFD0100600)。
