MST4、TRAF6及NF-κB在胎膜早破合并宫内感染患者中的表达及临床意义

The expression and clinical significance of MST4,TRAF6 and NF-κB in premature ruptured of membranes with intrauterine infection

目的探讨丝氨酸/苏氨酸蛋白激酶4(MST4)、肿瘤坏死因子受体相关因子6(TRAF6)及核因子-κB(NF-κB)在胎膜早破(PROM)合并宫内感染患者中的表达及其对早期宫内感染的诊断价值。方法选取2017年9月至2018年9月在本院分娩的30例正常孕妇为对照组,90例PROM孕妇为观察组。采用HE染色法对胎膜行病理学检查,ELISA测定孕妇外周血、脐血及羊水中的TRAF6、MST4及NF-κB的表达,ROC曲线分析三者对胎膜早破的诊断价值。结果PROM组90例中合并宫内感染54例,未合并宫内感染36例。PROM合并宫内感染组母血、脐血、羊水中TRAF6、MST4及NF-κB表达高于对照组和PROM未合并宫内感染组(P<0.05)。母血、脐血及羊水中TRAF6、MST4及NF-κB表达互呈正相关。ROC曲线分析提示NF-κB、MST4及TRAF6预测宫内感染价值最高的标本来源分别为母血、脐血、羊水。结论TRAF6、MST4及NF-κB可能参与PROM合并宫内感染的发生与发展,为宫内感染的早期诊断提供依据。

Objective To investigate the expression of serine/threonine protein kinase 4(MST4),tumor necrosis factor receptor-related factor 6(TRAF6)and nuclear factor-kappa B(NF-κB)in patients with premature rupture of membranes(PROM)complicated with intrauterine infection and their clinical value in the diagnosis of early intrauterine infection.Methods 30 normal pregnant women delivered in our hospital from September 2017 to September 2018 were selected as the control group and 90 PROM pregnant women as the experimental group.The fetal membranes were examined by hematoxylin-eosin(HE)staining.The expressions of TRAF6,MST4 and NF-κB in maternal peripheral blood,umbilical cord blood and amniotic fluid were detected by enzyme linked immunosorbent assay(ELISA)and receiver operating characteristic(ROC)curve was used to analyse the diagnostic value of the three indicators.Results There were 54 cases of intrauterine infection in the PROM group and 36 cases of no intrauterine infection in the PROM group.ELISA results showed that the expression of TRAF6,MST4 and NF-κB in PROM with intrauterine infection group was higher than those in control group and PROM without intrauterine infection group(P<0.05).The expressions of TRAF6,MST4 and NF-κB in maternal blood,umbilical cord blood and amniotic fluid were positively correlated.ROC curve analysis showed that NF-κB,MST4 and TRAF6 had the highest value in predicting intrauterine infection in maternal blood,umbilical cord blood and amniotic fluid,respectively.Conclusions TRAF6,MST4 and NF-κB may be involved in the occurrence and development of PROM complicated with intrauterine infection and provide evidence for the early diagnosis of intrauterine infection.