FBXO31对人宫颈癌细胞C-33A增殖的影响及其在人宫颈癌组织中的表达和临床意义研究

Effect of FBXO31 on Proliferation of Human Cervical Cancer Cell C-33A and Study on the Expression and Clinical Significance of FBXO31 in Human Cervical Cancer

目的:分析FBXO31(F-box only protein 31)对人宫颈癌细胞C-33A增殖的影响及其在人宫颈癌组织中的表达与临床因素间的相关性,探究其在人宫颈癌中的作用,以期寻找新的分子靶点。方法:MTT法分别检测不同浓度、不同时间时FBXO31作用下的C-33A细胞增殖抑制率。免疫组织化学法及蛋白质印迹(Western blotting)法检测70例人宫颈癌组织及癌旁组织中FBXO31的表达,并分析其与宫颈癌临床病理特征间的关系。结果:MTT法示,FBXO31作用后,C-33A细胞增殖受到抑制,且呈现浓度和时间依赖性。免疫组织化学法示,FBXO31主要表达于细胞核,人宫颈癌组织中FBXO31的阳性表达率(17/70,24.29%)明显低于癌旁组织(60/70,85.71%),差异有统计学意义(χ2=74.035,P<0.001)。Western blotting法示,人宫颈癌组织中FBXO31表达量明显低于癌旁组织,差异有统计学意义(P<0.05);进一步分析示FBXO31在宫颈癌组织中的表达与年龄、肿瘤大小及病理学类型无关,而与国际妇产科联盟(FIGO)分期、肿瘤分化程度及淋巴结转移和远处转移有关(P<0.05)。结论:FBXO31可能作为一种抑癌基因而抑制人宫颈癌C-33A细胞增殖,且呈时间及浓度依赖性。其在人宫颈癌组织呈低表达,且与宫颈癌临床特征关系密切,可能成为宫颈癌治疗新的分子靶点。

Objective:To analyze the effect of FBXO31(F-box only protein 31)on the proliferation of human cervical cancer cell C-33a and the correlation between its expression in human cervical cancer tissues and clinical factors,and to explore its role in the progression of human cervical cancer in order to find new molecular targets.Methods:MTT method was used to detect the inhibition rate of C-33A cells under the action of FBXO31 at different concentration and time.The expression of FBXO31 in cervical cancer tissues and adjacent tissues of 70 patients were detected by immunohistochemistry and Western blotting,and the relationship between FBXO31 expression and clinical pathological features was analyzed.Results:MTT method showed that the proliferation of C-33A cells was inhibited by FBXO31,and the proliferation inhibition rate showed a concentration and time-dependent.Immunohistochemistry showed that FBXO31 was mainly expressed in the nucleus.The positive expression rate of FBXO31 in cervical cancer tissues(17/70,24.29%)was significantly lower than that in the adjacent tissues(60/70,85.71%)(χ2=74.035,P<0.001).Western blotting showed that the expression of FBXO31 in human cervical cancer tissues was obviously lower than that in adjacent tissues,with statistically significant difference(P<0.05).Further analysis showed that the expression of FBXO31 in cervical cancer tissues was not related to age,tumor size and pathological type,but to FIGO stage,tumor differentiation degree,lymph node metastasis and distant metastasis(P<0.05).Conclusions:FBXO31 may inhibit the proliferation of human cervical cancer C-33A cells as a tumor suppressor gene in a time and concentration dependent manner.It has low expression in human cervical cancer tissues and is closely related to the clinical characteristics of cervical cancer,which may be a new molecular target for cervical cancer treatment.