RNA结合蛋白6对喉癌细胞增殖、迁移和凋亡的影响

Effect of RNA binding protein 6 on proliferation,migration and apoptosis of laryngeal cancer cells

目的观察RNA结合蛋白6(RBM6)对喉癌细胞增殖、迁移和凋亡的影响。方法收集2016年6月到2018年8月驻马店市中心医院喉癌组织和癌旁组织47例,采用蛋白质印迹法(Western blot)分析RBM6蛋白的表达水平。构建RBM6过表达慢病毒载体,包装慢病毒,感染Hep-2细胞,构建RBM6过表达细胞株和对照组细胞株(RBM6组和对照组)。采用细胞计数试剂盒(CCK-8)分析两组细胞增殖;采用划痕实验分析两组细胞的迁移;采用流式细胞术分析两组细胞增殖;采用异体移植瘤实验分析肿瘤细胞在体内的生长。应用SPSS 13.0统计软件分析,计量数据采用均值±标准差(Mean±SD)表示,组间比较采用t检验。结果与癌旁组织RBM6蛋白水平(1.01±0.23)比较,喉癌组织中RBM6蛋白表达水平(0.34±0.12)显著下调,差异有统计学意义(t=2.913,P<0.05)。与对照组细胞比较,RBM6组细胞增殖能力显著下降,差异有统计学意义(F=3.019,P<0.05)。与对照组细胞划痕愈合率[(84.18±8.32)%]比较,RBM6组细胞愈合率[(30.11±6.89)%]显著下降,差异有统计学意义(t=4.319,P<0.05)。与对照组细胞凋亡水平[(35.61±7.01)%]比较,RBM6组细胞凋亡水平[(5.23±2.12)%]显著增加,差异有统计学意义(t=2.192,P<0.05)。体内增殖实验结果显示,对照组细胞在裸鼠体内增殖速度明显高于RBM6组细胞,差异有统计学意义(F=2.908,P<0.05)。结论RBM6抑制喉癌细胞的增殖和迁移,促进肿瘤细胞凋亡。

Objective To investigate the effect of RNA binding protein 6(RBM6)on the proliferation,migration and apoptosis of laryngeal cancer cells.Methods The expression of RBM6 protein was analyzed by Western blotting in 47 cases of laryngeal cancer and adjacent tissues.RBM6 overexpression cell line and control cell line(RBM6 group and control group)were constructed by RBM6 overexpression lentivirus and control lentivirus respectively.The proliferation of two groups was analyzed by cell counting kit-8(CCK-8).The migration was analyzed in RBM6 group and control group by scratch test.The apoptosis level in RBM6 group and control group was analyzed by flow cytometry.The growth of tumor cells in vivo in RBM6 group and control group was analyzed by allogeneic tumor transplantation.SPSS 13.0 statistical software was used to analyze the measurement data.Mean±SD was used to express the measurement data,and t-test was used to compare between groups.Results As compared with the level of RBM6 protein in adjacent tissues(1.01±0.23),the expression level of RBM6 protein(0.34±0.12)in laryngeal cancer significantly decreased(t=2.913,P<0.05).As compared with the control group,the cell proliferation ability of RBM6 group significantly decreased(F=3.019,P<0.05).As compared with the control group[(84.18±8.32)%],Scratch healing rate in RBM6 group[(30.11±6.89)%]significantly enhanced(t=4.319,P<0.05).As compared with the control group[(35.61±7.01)%],the apoptosis rate in RBM6 group[(5.23±2.12)%]significantly increased(t=2.192,P<0.05).The results showed that the proliferation rate of cells in the control group was significantly higher than that in the RBM6 group(F=2.908,P<0.05).Conclusion RBM6 can inhibit the proliferation and migration of laryngeal cancer cells,and promote the apoptosis of tumor cells.