X连锁犰狳重复蛋白3在甲状腺乳头状癌组织的表达及其临床意义

Expression and clinical significance of armadillo repeat-containing X-linked protein 3 in papillary thyroid carcinoma

目的探讨甲状腺乳头状癌中X连锁犰狳重复蛋白3(ALEX3)的表达及意义。方法收集郑州大学第一附属医院2018年6月至2018年9月60例甲状腺乳头状癌患者(男10例,女50例)手术新鲜癌组织及正常甲状腺组织,应用实时定量聚合酶链反应(Real-time PCR)技术和蛋白免疫印迹法(Western blot)检测甲状腺乳头状癌组织及配对正常甲状腺组织中ALEX3 mRNA和蛋白的表达,以ALEX3表达水平的中位值作为截断值,将60例甲状腺癌乳头状癌患者分为ALEX3高表达组和ALEX3低表达组。采用t检验分析甲状腺乳头状癌组织和正常甲状腺组织ALEX3表达量差异,采用χ^2检验分析ALEX3 mRNA表达水平与甲状腺癌患者临床病理学特征的相关性。结果ALEX3 mRNA在甲状腺乳头状癌组织相对表达量(2.125±0.755)显著高于正常甲状腺组织(1.156±0.426,t=8.663,P<0.05),差异有统计学意义,其表达水平与TNM分期和淋巴结转移密切相关(χ^2=5.963、15.152,P<0.05)。ALEX3蛋白在甲状腺乳头状癌组织相对表达量(0.332±0.145)明显高于正常甲状腺组织(0.204±0.132,t=2.252,P<0.05),差异有统计学意义。结论ALEX3在甲状腺乳头状癌组织中显著高表达,可能在其发生、发展中起重要作用。

Objective To explore the expression and significance of armadillo repeat-containing X-linked protein 3(ALEX3)in papillary thyroid carcinoma.Methods From June 2018 to September 2018 a total of 60 cases(10 males and 50 females)of papillary thyroid carcinoma in the First Affiliated Hospital of Zhengzhou University were collected.Real-time quantitative polymerase chain reaction(Real-time PCR)and Western blotting were used to detect the expression levels of ALEX3 in papillary thyroid carcinoma tissues and normal thyroid tissues,respectively.The median value of the ALEX3 expression level is used as the truncated value,60 patients with papillary thyroid cancer were divided into the ALEX3 high-expression group and the ALEX3 low-expression group.The t test was used to analyze the difference in ALEX3 expression level between thyroid papillary carcinoma tissues and normal thyroid tissues,and theχ^2 test was used to analyze the correlation between ALEX3 mRNA and clinicopathological features in papillary thyroid carcinoma.Results The relative expression value of ALEX3 mRNA in papillary thyroid carcinoma tissues(2.125±0.755)was significantly higher than that in normal thyroid tissues(1.156±0.426,t=8.663,P<0.05).The difference was statistically significant.Expression level of ALEX3 mRNA was closely related to TNM pathological stage and lymph node metastasis(χ^2=5.963,15.152,P<0.05).The relative expression value of ALEX3 protein in papillary thyroid carcinoma tissues(0.332±0.145)was significantly higher than that in normal thyroid tissues(0.204±0.132,t=2.252,P<0.05),The difference was statistically significant..Conclusion ALEX3 was significantly over-expressed in thyroid papillary carcinoma,and might play an important role in its occurrence and progression.