SAHA对HeLa细胞增殖、凋亡、衰老以及迁移的影响

Effects of SAHA on the Proliferation,Apoptosis,Aging and Migration of HeLa Cells

为研究HDAC抑制剂SAHA对宫颈癌HeLa细胞肿瘤生物学特性的影响,使用SAHA处理HeLa细胞后,在显微镜下观察细胞形态,台盼蓝染色法检测细胞增殖,TUNEL法检测细胞凋亡,β-半乳糖苷酶染色法检测细胞衰老,划痕实验和小室迁移实验检测细胞迁移能力,实时荧光定量PCR(Real-time PCR)和免疫印迹(Western blot)检测基因表达.结果显示:SAHA处理HeLa细胞后,细胞增殖能力下降,发生衰老,同时在分子水平上促进p21基因表达;抑制细胞迁移,在分子水平上迁移相关基因MYL9表达水平下降;SAHA处理的细胞没有表现出明显凋亡现象.本研究结果提示SAHA可能通过促进p21和MYL9基因表达发挥抑制细胞增殖、促进细胞衰老和降低细胞迁移能力的作用.

To study the effects of SAHA on the biological characteristics of cervical cancer cells,HeLa cells were treated with SAHA and then the morphological changes were investigated under microscope.Cell proliferation was determined with trypan blue staining assay.Apoptosis was examined with TUNEL staining.Cell senescence was tested withβ-galactosidase staining.Cell migrating ability was examined with wound-healing assay and transwell migration assay.Real-time PCR and Western blot were used to measure p21 and MYL9 expression.The results showed that the treatment of HeLa with SAHA could not only inhibit cell proliferation and cause the cell senescence but also increase the p21 gene expression.No apoptosis was observed in HeLa cells following SAHA treatment.Additionally,cell migration ability was undermined,which was consistent with the down-regulation of the migration-related gene MYL9.The above results indicate that,by promoting p21 and inhibiting MYL9 gene expression,SAHA may inhibit cell proliferation,promote cell senescence and reduce cell migration.