氟唑菌酰羟胺和戊唑醇对假禾谷镰刀菌联合毒力测定

Joint-toxicity Measurement of Pydiflumetofen and Tebuconazole to Fusarium pseudogramineaqum

为探明氟唑菌酰羟胺、戊唑醇对假禾谷镰刀菌联合毒力作用,进行高效药剂筛选,为设计药剂防治方案提供理论依据。本研究采用常规组织分离法,从泰安及周边地区病样上分离到假禾谷镰刀菌(Fusarium pseudogramineaqum)菌株,进行致病性测定;采用菌丝生长速率法,测定氟唑菌酰羟胺、戊唑醇2种杀菌剂对假禾谷镰刀菌的室内生物活性。结果表明,氟唑菌酰羟胺、戊唑醇对假禾谷镰刀菌的EC50分别为0.107、0.906 mg/L,说明氟唑菌酰羟胺对假禾谷镰刀菌的室内生物活性明显优于戊唑醇。在氟唑菌酰羟胺与戊唑醇的5个不同配比中,1:1配比的EC50最小,为0.157 mg/L;1:8配比的EC50最大,为0.303 mg/L。氟唑菌酰羟胺与戊唑醇的5个不同配比中的SR分别为1.22、1.54、1.85、1.98、1.63,其中,1:2、1:4、1:6、1:8混配组合的SR均大于1.5,表明上述4种混配组合对假禾谷镰刀菌均表现为增效作用,且1:6的增效作用最强。

To explore the joint-toxicity of pydiflumetofen and tebuconazole to Fusarium pseudogramineaqum,carry out the selection of efficient medicament,and provide a theoretical foundation for designing drug prophylaxes,through the conventional tissue isolation,Fusarium pseudogramineaqum strains were isolated from infected samples in Tai’an and surrounding areas,and the pathogenicity was measured.The indoor bioactivities of pydiflumetofen and tebuconazole to Fusarium pseudogramineaqum were detected by mycelium growth rate method.The results showed that EC50 of pydiflumetofen and tebuconazole to Fusarium pseudogramineaqum was 0.107 mg/L and 0.906 mg/L,respectively,which indicated that the indoor bioactivity of pydiflumetofen was better than that of tebuconazole.Among the five ratios of pydiflumetofen and tebuconazole,the minimum EC50 was found at ratio 1:1(0.157 mg/L),and the maximum EC50 was found at ratio 1:8(0.303 mg/L).The SRs of these five ratios were 1.22,1.54,1.85,1.98 and 1.63,among which the SRs of ratio 1:2,1:4,1:6 and 1:8 were greater than 1.5.It indicated that all these four ratios had synergistic effect on Fusarium pseudogramineaqum,and the effect of ratio 1:6 was the most obvious.