不同生长时期裕丹参微量元素动态变化

Dynamic Changes of Trace Elements in Yu Salvia miltiorrhiza in Different Growth Periods

目的:建立原子吸收光谱法测定不同生长时期裕丹参K,Cu,Na,Mg,Fe,Zn,Ca,Mn 8种微量元素含量,探讨裕丹参在生长过程中微量元素含量变化规律和药材质量的关系。方法:采用微波消解-原子吸收光谱法测定不同生长时期裕丹参根和生长环境土壤中的微量元素,借用高效液相色谱法进行指标成分含量测定,运用主成分分析、相关性分析方法对结果进行统计和评价。结果:裕丹参在不同生长时期各微量元素含量存在明显差异,不同生长期与Cu存在显著正相关,与Zn,Ca,Mn存在显著负相关,一年生裕丹参质量综合得分最优;指标成分含量与Mn呈极显著负相关,与Zn,Ca呈显著负相关,与Cu,Fe,Na呈正相关;土壤中Mg,Fe,Ca,Mn与药材中Zn,Ca,Mn均呈相关趋势,Mn与药材中Cu呈极显著负相关趋势;药材中K,Mg含量随生长时期变化逐渐增加且一年生裕丹参总体得分为最优。结论:不同时期裕丹参微量元素变化具有一定规律性,土壤中微量元素影响药材微量元素发生变化,药材中微量元素与指标成分关系密切,与药材质量具有一定的关系。

Objective:To establish an atomic absorption spectrometry to determine the content of eight trace elements of Yu Salvia miltiorrhiza in different growth stages,namely K,Cu,Na,Mg,Fe,Zn,Ca and Mn.Method:Micro-digestion-atomic absorption spectrometry was used to determine the trace elements in the roots of Yu S.miltiorrhiza.The HPLC method was used to determine the content of active constituents of Yu S.miltiorrhiza.Principal component analysis and correlation analysis were used to evaluate the results.Result:The contents of trace elements in Yu S.miltiorrhiza in different growth periods were significantly different.Cu had a significant positive correlation with the growth period,while Zn,Ca and Mn had significant negative correlations with the growth period.The comprehensive score of Yu S.miltiorrhiza in December was the best.The content of index components was negatively correlated with Mn,Zn and Ca,and positively correlated with Cu,Fe and Na.In soil,Mg,Fe,Ca and Mn were correlated with Zn,Ca and Mn,while Mn was negatively correlated with Cu.The content of K and Mg in the crude drug increased gradually with the change of the growth period,and the overall score of annual Yu S.miltiorrhiza was the best.Conclusion:The change of trace elements in Yu S.miltiorrhiza in different periods has certain regularity.Trace elements in soil have impacts on trace elements in medicinal materials.Trace elements in medicinal materials are closely correlated with index components and quality of medicinal materials.