68个大豆品种(系)遗传多样性分析

Genetic diversity analysis of 68 soybean varieties

为保持大豆杂种优势利用中亲本的遗传差异,利用64对SSR引物对68个大豆品种(系)包括53个中国品种(系)和15个美国品种进行遗传多样性与亲缘关系分析。结果表明,共检测到375个等位基因变异,平均每个位点检测到的等位变异数为5.86个,变化范围为2~12个,其中53个中国品种(系)检测到373个等位基因变异,平均为5.83个,变化范围为2~12个,15个美国品种检测到288个等位基因变异,平均为4.50个,变化范围为2~9个;总的位点多态性信息含量(PIC)变化范围为0.366~0.900,平均为0.677,其中53个中国品种(系)变化范围为0.370~0.909,平均为0.682,15个美国品种变化范围为0.309~0.817,平均为0.590。聚类分析表明:68个大豆品种(系)的遗传相似系数(GS)变幅为0.529~0.973,平均GS值0.707,在相似系数0.65处,可将供试的品种(系)聚为2大类,第Ⅱ类在相似系数0.69处又可分为5个亚类。

In order to maintain the genetic differences of parents in the utilization of soybean heterosis,64 pairs of SSR primers were used to analyze the genetic diversities and genetic relationships of 68 soybean varieties(lines),including53 Chinese varieties(lines)and 15 American varieties.The results showed that:A total of 375 allele variations were detected,with an average of 5.86 alleles per locus,ranging from 2 to 12.Among them,373 allele variations were detected in 53 Chinese varieties(lines)with an average of 5.83,ranging from 2 to 12,and 288 allele variations were detected in 15 American varieties with an average of 4.50,ranging from 2 to 9.The PIC of polymorphism information content ranged from 0.366 to 0.900,with an average of 0.677.Among them,53 Chinese varieties(lines)ranged from0.370 to 0.909,with an average of 0.682,and 15 American varieties ranged from 0.309 to 0.817,with an average of0.590.The results of cluster analysis showed that the genetic similarity coefficient(GS)of 68 soybean varieties(lines)varied from 0.529 to 0.973,with an average GS value of 0.707.In conclusion,at the similarity coefficient of0.65,the tested varieties(lines)could be clustered into two groups,of which the second group could be divided into five subgroups at the similarity coefficient of 0.69.