联苯菊酯对菲律宾蛤仔肝胰腺细胞DNA损伤的初探

Primary study on DNA damage in hepatopancreas cells of Ruditapes philippinarum exposed to bifenthrin

为了评价联苯菊酯的遗传毒性,研究了不同浓度联苯菊酯对菲律宾蛤仔肝胰腺细胞DNA的损伤作用。研究分别在0.05、0.10、0.15、0.20 mg/L联苯菊酯暴露25 d后,取菲律宾蛤仔肝胰腺细胞并用单细胞凝胶电泳(彗星实验)进行分析,以彗尾DNA相对含量(TDNA%)、彗星尾长(TL)、Olive尾矩(OTM)和拖尾率(TR)作为DNA损伤的指标,实验结果表明:与空白对照组相比,各处理组菲律宾蛤仔肝胰腺细胞DNA均有不同程度的损伤且各检测指标值均显著增加(P<0.05);随着联苯菊酯浓度的增加,各检测指标均呈规律性的增长趋势,具有高度的相关性(r>0.99),表现出良好的剂量效应关系。评价彗星实验较好的检测指标为彗尾DNA相对含量和Olive矩,实验获得的多元回归方程具有显著的统计学意义(P<0.01),可以有效地推断联苯菊酯对菲律宾蛤仔的染毒毒性浓度。

To evaluate the genotoxicity of bifenthrin,the present study investigated the damage effects of different concentrations of bifenthrin on the DNA of hepatopancreas cells in Ruditapes philippinarum.After exposure to 0.05,0.10,0.15,0.20 mg/L bifenthrin for 25 days,hepatopancreas cells of Ruditapes philippinarum were extracted and assayed by using the single cell gel-electrophoresis(SCGE).The percentage of comet tail DNA(TDNA%),comet tail length(TL),olive tail moment(OTM)and Tail rate(TR)were used as the indicators of DNA damage.The results showed that,all indicators in all treatment groups increased significantly compared with those in the control group(P<0.05).All indicators increased gradually according to the increased concentrations of bifenthrin.The correlation coefficient of r was greater than 0.99,which indicated that the concentration-effect relationship was observed in the experimental concentration range.TDNA%and OTM were the better experimental indices in the assessment of comet assay.The multiple-dimensional regression equation had significant statistic meaning(P<0.01),which might be an effective way to estimate the exposure concentration of bifenthrin.