miR-199a和lncRNA SNHG12在宫颈癌中的表达水平、相关性及临床意义

Expression levels,correlations,and clinical significance of miR-199a and lncRNA SNHG12 in cervical cancer

目的研究微小RNA 199a (miR-199a)和长链非编码RNA小核宿主基因12 (lncRNA SNHG12)在宫颈癌中的表达水平、相关性及临床意义.方法选取2012年6月-2014年12月该院病理存档的220例宫颈组织标本及病理资料为研究对象,其中宫颈炎75例(对照组),宫颈上皮内瘤变(CIN) 71例(CIN组),宫颈癌74例(CC组).采用实时荧光定量PCR(RT-qPCR)检测各宫颈组织中miR-199a和lncRNA SNHG12表达,并分析其与宫颈癌临床病理参数的关系;采用Pearson法分析宫颈癌组织中二者表达关系;采用Kaplan-Meier生存曲线法分析宫颈癌患者5年生存率;采用COX分析影响宫颈癌患者预后的危险因素.结果对照组、CIN组、CC组患者宫颈组织中miR-199a表达水平依次降低,lncRNA SNHG12表达水平依次升高(均P>0.05).miR-199a、lncRNA SNHG12表达水平与宫颈癌患者病理类型、FIGO分期、淋巴结转移有关(均P<0.05),与患者年龄、分化程度、浸润深度无关(均P>0.05).宫颈癌患者癌组织中miR-199a、lncRNA SNHG12表达水平呈负相关(r=-0.518,P<0.05).miR-199a低表达组宫颈癌患者5年生存率为9.52%,显著低于miR-199a高表达组(75.47%),差异有统计学意义(x2=35.09,P<0.05);lncRNA SNHG12高表达组宫颈癌患者5年生存率为21.74%,显著低于lncRNA SNHG12低表达组(72.00%),差异有统计学意义(x2=17.45,P<0.05).COX多因素分析显示,miR-199a、lncRNA SNHG12表达水平及淋巴结转移是影响宫颈癌患者预后的独立危险因素(均P>0.05).结论宫颈癌组织中miR-199a低表达,lncRNA SNHG12高表达,二者呈负相关,且与宫颈癌患者5年生存率及预后有关,是影响宫颈癌患者预后的独立危险因素,有潜力成为监测宫颈癌病情发展及预后评估的有效指标.

Objective To study the expressions and clinical significances of micro RNA 199 a(miR-199 a)and long non-coding RNA small nucleus host gene 12(lncRNA SNHG12)in cervical cancer.Methods 220 cervical tissue specimens and pathological data from June 2012 to December 2014 were selected as the research objects,including 75 cases of cervicitis(control group),71 cases of cervical intraepithelial neoplasia(CIN group),and 74 cases of cervical cancer(CC group).Real-time fluorescence quantitative PCR(RT-q PCR)was used to detect the expressions of miR-199 a and lncRNA SNHG12 in cervical tissues,the relationship between cervical cancer and clinicopathological parameter was analyzed,Pearson method was used to analyze the relationship between the two expressions in cervical cancer tissues,Kaplan-Meier survival curve method was used to analyze the 5-year survival rate of cervical cancer patients,and COX was used to analyze the risk factors affecting the prognosis of patients with cervical cancer.Results The expression level of miR-199 a in cervical tissues of control group,CIN group and CC group decreased in turn,while the expression level of lncRNA SNHG12 increased in turn(all P<0.05).The expression levels of miR-199 a and lncRNA SNHG12 were correlated with the pathological type,FIGO stage and lymph node metastasis of cervical cancer patients(all P<0.05),but not with age,differentiation degree and infiltration depth(all P>0.05).The expression levels of miR-199 a and lncRNA SNHG12 in cervical cancer tissues were negatively correlated(r=-0.518,P<0.05).The 5-year survival rate of patients with cervical cancer in the low-expression group of miR-199 a was 9.52%,which was significantly lower than that in the high-expression group of miR-199 a(75.47%)(χ2=35.09,P<0.05).The 5-year survival rate of cervical cancer patients in the high expression group of lncRNA SNHG12 was 21.74%,which was significantly lower than that in the low expression group of lncRNA SNHG12(72.00%)(χ2=17.45,P<0.05).COX multivariate analysis showed that the expressions of miR-199 a,lncRNA SNHG12 and lymph node metastasis were independent risk factors affecting the prognosis of cervical cancer patients(all P<0.05).Conclusion The expression of miR-199 a is low and the expression of lncRNA SNHG12 is high in cervical cancer,they are negatively correlated,and correlated with the5-year survival rate and prognosis of cervical cancer patients.They are independent risk factors affecting the prognosis of patients with cervical cancer,and have the potential to become effective indicators for monitoring the development of cervical cancer and evaluating its prognosis.