Notch1在皮肤黑色素瘤血管生成中的作用研究

Role of Notch1 in angiogenesis of cutaneous melanoma

目的探讨Notch1在皮肤黑色素瘤血管生成中的作用。方法采用免疫组织化学SP法检测78例皮肤黑色素瘤组织和40例皮内痣组织中Notch1、血管内皮生长因子(VEGF)的表达,以及CD34标记的微血管密度(CD34-MVD)。结果皮肤黑色素瘤组织中Notch1阳性表达率、VEGF阳性表达率及CD34-MVD均高于皮内痣组织,差异有统计学意义(P<0.05)。皮肤黑色素瘤组织中Notch1表达与VEGF表达和CD34-MVD均呈正相关(P<0.05);皮肤黑色素瘤组织中Notch1阳性表达率、VEGF阳性表达率、CD34-MVD与Clark分级、淋巴结转移有关(P<0.05)。结论Notch1和VEGF可能在皮肤黑色素瘤血管生成中具有一定的协同作用,并在肿瘤转移浸润过程中共同发挥作用。

Objective To explore the role of Notch1 in angiogenesis of cutaneous melanoma.Methods Immunohistochemical SP method was performed to detect the expressions of Notch 1 and vascular endothelial growth factor(VEGF)in 78 cases of cutaneous melanoma and 40 cases of intradermal nevus,as well as the CD34 marked microvessel density(CD34-MVD).Results The positive expression rates of Notch1,VEGF and CD34-MVD were higher in cutaneous melanoma tissues than in intradermal nevus tissues,with statistically significant differences(P<0.05).The expression of Notch1 was positively correlated with the expression of VEGF and CD34-MVD in cutaneous melanoma tissues(P<0.05).The expression of Notch1,VEGF and CD34-MVD in cutaneous melanoma tissues was related to Clark classification and lymphatic metastasis(P<0.05).Conclusion Notch1 and VEGF may play a synergistic role in melanoma angiogenesis,and also play a role in tumor metastasis and invasion.