Polo样蛋白激酶1抑制剂BI-2536对人肝癌SNU-423细胞增殖及侵袭的影响

Effect of BI-2536,an inhibitor of Polo-like protein kinase 1,on proliferation and invasion of human hepatocellular carcinoma SNU-423 cells

目的探讨Polo样蛋白激酶1(Polo-like protein kinase 1,PLK1)抑制剂BI-2536对人肝癌SNU-423细胞增殖及侵袭的影响。方法体外培养人肝癌SNU-423细胞,采用不同浓度梯度的BI-2536作用于SNU-423细胞,同时设对照组(未加BI-2536),MTT法检测BI-2536对人肝癌SNU-423细胞的抑制率,划痕试验检测BI-2536对人肝癌SNU-423细胞侵袭和迁移能力的影响。结果SNU-423细胞经BI-2536处理48 h后的IC50为5.036μmol/L。SNU-423细胞的增殖抑制率随BI-2536浓度的增加明显上升(P<0.01);经BI-2536作用的SNU-423细胞迁移率明显低于对照组(P<0.01),随药物浓度的增高,SNU-423细胞出现凋亡现象。结论BI-2536可抑制人肝癌SNU-423细胞的增殖及迁移能力,PLK1可作为肝癌治疗中的新靶点。

Objective To investigate the effect of BI-2536,an inhibitor of Polo-like protein kinase 1,on the proliferation and invasion of human hepatocellular carcinoma SNU-423 cells.Methods Human hepatocellular carcinoma SNU-423 cells were cultured in vitro,and treated with BI-2536 at various concentration gradients,using those untreated as control.The inhibitory rate of BI-2536 to SNU-423 cells was determined by MTT assay,while the effects on invasion and migration abilities by scratch test.Results The IC50 of SNU-423 cells after treatment with BI-2536 for 48 h was 5.036μmol/L.The inhibitory rate of proliferation of SNU-423 cells increased significantly with the increasing BI-2536 concentration(P<0.01).The migration rate of SNU-423 cells treated with BI-2536 was significantly lower than that in control group(P<0.01).However,with the increasing concentration of BI-2536,the apoptosis of SNU-423 cells appeared.Conclusion BI-2536 inhibited the proliferation and migration of human hepatocellular carcinoma SNU-423 cells,and PLK1 might be used as a novel target in the treatment of hepatocellular carcinoma.