黄海希瓦氏菌胞外产物特性研究

Characterization of Extracelluar Production of Shewanella smarflavi

为研究黄海希瓦氏菌(S.smarflavi)AP629胞外产物(ECPs)的致病性,用平板玻璃纸法提取胞外产物。结果显示,SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)发现ECPs蛋白条带主要在114 kDa、66 kDa、39kDa、36 kDa、79 kDa;ECPs具有酪蛋白酶、淀粉酶、卵磷脂蛋白酶和脂肪酶活性,无明胶酶活性;一些金属离子和化学试剂对菌株AP629ECPs的酶活有影响,EDTA、PMSF、SDS、MnCl2作用浓度分别为10、5、5、5 mM时,ECPs酶活受到不同程度抑制;Ca2+和Mg2+则提高ECPs的酶活,可分别提高3%~19%和12%~28%的酶的活性。菌株AP629的胞外产物人工感染小白鼠和仿刺参试验结果表明,黄海希瓦氏菌AP629的胞外产物不显示致病性。该文为揭示黄海希瓦氏菌AP629发病机理奠定了基础。

In order to study the pathogenicity of ECPs from Shewanella smarflavi,ECPs of S.smarflavi AP629 was extracted by the glass and paper method.Results showed:SDS-PAGE of ECPs proteins bands(114 kDa、66 k Da、39 kDa、36 kDa、79 kDa);the ECPs had caseinolytic,amylase,protease and lipase activity,while an absence of gelatinase activity;and some metalions and chemical reagents had influences on the activity of pathogens ECPs,and when the effect concentrations of EDTA,PMSF,SDS and MnCl2 were 10 m M,5 m M,5 m M,5 m M,ECPs activity was inhibited in various degree;and the enzyme activity of ECPs was increased by Ca2+and Mg2+,3~19%and 12~28%,respectively.The results of ECPs pathogenicity for mice and Apostichopus japonicus demonstrated that ECPs were no virulent properties for S.smarflavi AP629.All these work provides guidance and foundation for exploring pathogenesis of S.smarflavi AP629.