CC趋化因子配体8在胶质瘤组织中的表达及其临床意义

Expression of CCL8 in the glioma and its clinical significance

目的探究CC趋化因子配体8(CCL8)在人脑胶质瘤组织中的表达情况及其临床意义。方法从癌症基因组图谱(TCGA)数据库收集697例胶质瘤数据集,下载基因表达谱资料和临床信息资料。分析CCL8 mRNA表达与胶质瘤WHO分级、分子分型的关系;根据CCL8 mRNA的表达水平将TCGA数据库中的胶质瘤样本分为CCL8 mRNA低表达组和CCL8 mRNA高表达组,采用Kaplan-Meier法绘制生存曲线,比较2组总生存时间。结果CCL8 mRNA在胶质母细胞瘤(WHOⅣ级)组织中的表达水平高于低级别胶质瘤(WHOⅡ、Ⅲ级)组织(P<0.001);CCL8 mRNA在间质型胶质瘤组织中的表达高于经典型、神经元型、原神经细胞型胶质瘤组织(P<0.001);CCL8 mRNA低表达组的中位生存时间为4068 d,显著高于CCL8 mRNA高表达组的954 d(P<0.001)。结论CCL8 mRNA的表达与胶质瘤的发生、发展相关,提示CCL8可能成为一个潜在的胶质瘤治疗靶点。

Objective To explore the expression of CC chemokine ligand 8(CCL8)in the glioma tissues,and to explore its clinical significance.Methods A total of 697 glioma datasets were collected from the Cancer Genome Atlas(TCGA)database,and the gene expression profiles and clinical information were downloaded.Then the relationship between CCL8 mRNA expression and WHO grading or molecular typing in the glioma were analyzed.The glioma samples in the TCGA database were divided into the CCL8 mRNA low expression group and the CCL8 mRNAhigh expression group according to the expression level of CCL8 mRNA,Kaplan-Meier method was used to draw the survival curve,and the overall survival time of the two groups were compared.Results In the TCGA database,the expression of CCL8 in the glioblastoma(WHO gradeⅣ)was significantly accumulated compared with low grade glioma(WHO gradeⅡ,Ⅲ)at mRNA level(P<0.001).The expression of CCL8 in the mesenchymal glioma was significantly higher than that in the classical,neuronal,and proneural glioma(P<0.001).The median survival time of the CCL8 low expression group and the CCL8 high expression group was 4068 days and 954 days,respectively,and the difference was significant(P<0.001).Conclusion CCL8 mRNA expression is related with the occurrence and development of glioma,suggesting that CCL8 may serve as a potential therapeutic target for glioma.