枸芪复肾丸对IgA肾病模型大鼠Th1/Th2影响的研究

Influences of Gouqi Fushen pills for Th1/Th2 of IgA nephropathy model rats

目的探讨枸芪复肾丸对IgA肾病的疗效及对IgAN模型大鼠Th1/Th2的影响。方法 Wistar大鼠72只,随机分为空白组、枸芪复肾丸高、中、低剂量组、洛汀新组、模型组。采用BSA口服和LPS尾静脉注射法建立IgAN大鼠模型。8周后观察大鼠各时相UTP、尿红细胞;检测血清IL-2、INF-γ、IL-4、IL-10。结果治疗组大鼠UTP及尿RBC均下降,枸芪复肾丸中剂量组优于洛汀新组(P<0.05);给药后治疗组大鼠IL-2、INF-γ升高,IL-4、IL-10下降,Th1/Th2失衡状态明显纠正(P<0.05)。结论枸芪复肾丸能有效减轻IgA肾病大鼠蛋白尿、血尿;IgA肾病模型大鼠经给药后IL-2、INF-γ升高,IL-4、IL-10下降,纠正Th1/Th2失衡。

Objective To investigate the Effects of Gouqi Fushen pills for IgAN and to discuss influences of Gouqi Fushen pills for Th1/Th2 of IgAN model rats.Methods 72 rats were randomly divided into blank group,Gouqi Fushen pills low,middle and high dose group,lotensin group(control group),model group.The model of IgAN rats were established by the method of feeding with bovine serum albumin(BSA)and injecting tail vein with lipopolysaccharide(LPS).Eight weeks treatment later,each phase’s UTP,urine red blood cell count,expression level of serum cytokines IL-2,IL-4,IL-10,interference prime(INF)–γwere observed.Results After treatment,urine red blood cell count and UTP of lotensin group rats,Gouqi Fushen pills low,middle and high dose group rats were decreased.Compared with lotensin group,Gouqi Fushen pills middle dose group decreased extraordinary(P<0.05);Compared with the blank group,the serum IL-2 and INF-γof model group rats were significantly lower(P<0.05)and IL-4,IL-10 were significantly increased(P<0.05)and Th1/Th2 ratio appears to drift.Compared with model group,the serum IL-2 and INF-γof the treatment group rats increased(P<0.05)and IL-4,IL-10 levels decreased(P<0.05)and Th1/Th2 drift improved(P<0.05).Conclusion Gouqi Fushen pills can reduce the IgAN rats hematuria and proteinu-ria,and it is better than Lotensin.Gouqi Fushen pills can adjust cytokines IL-2,IL-4,IL-10 and INF-γ,and correct the Th1/Th2 ratio drift.