气雾培下酸处理马铃薯植株结薯特点及叶片生理生化指标的变化

Minituberization Characteristics of Potato Plant and Physiological and Biochemical Indexes in Its Leaf with Acid Treatment at Aeroponics

用磷酸水溶液对马铃薯植株根际进行酸处理,调查酸处理植株(AT)的结薯特性,测定微型薯形成过程中叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性及丙二醛(MDA)和脯氨酸(Pro)的含量。结果表明:p H 3处理3 d的AT植株平均每株结薯量最高,比对照增加了4.8倍,结薯始期比对照提前12 d。诱导微型薯过程中,AT和对照叶片SOD、CAT活性和Pro含量变化趋势相似;相同测定时间的AT和对照叶片中SOD、POD和CAT活性及MDA和Pro含量存在差异。诱导微型薯1 d时,AT叶片的SOD和CAT活性恢复到对照水平,而POD活性显著低于对照,MDA和Pro含量显著高于对照。诱导微型薯5 d后,AT叶片的SOD、POD、CAT活性始终显著高于对照,而MDA和Pro含量始终显著低于对照。

The rhizosphere of potato plants was treated with phosphoric acid aqueous solution.The characteristics of potato minituberization were investigated.The changes of antioxidant enzyme activities of superoxide dismutase(SOD),peroxidase(POD)and hydrogen peroxidase(CAT)and contents of malondialdehyde(MDA)and proline(Pro)were measured in leaves of plants with acid treatment(AT)and without acid treatment(Control)during potato minituber formation at aeroponics.The results showed that the highest mean number of minituber was found in the acid treatment by pH 3 for 3 d,4.8 times higher than control,and the initial stage of minituber formation in AT was 12 days earlier than control.During minituberization,the SOD and CAT activities and the Pro content had similar changing trends in leaves of AT and control.The activities of SOD,POD and CAT and the contents of Pro and MDA were different in leaves of both AT and control at the same culture time.On the first day of tuber induction,the activities of SOD and CAT in AT leaves returned to the level of control,and the POD activity was obviously lower than control,the contents of MDA and Pro were significantly higher than control.The activities of SOD,POD and CAT were significantly higher than control,and the contents of MDA and Pro were significantly lower than control after 5 days of potato tuberization.