摘要
为研究林蛙卵油的脂肪酸成分、多不饱和脂肪酸的富集工艺及氧化稳定性,将提取得到的东北林蛙卵油中的脂肪酸进行GC-MS检测,对尿素包合法分离纯化多不饱和脂肪酸进行正交试验,并将林蛙卵油在不同条件下储存0,6,12,18,24,30 d,测定各组的过氧化值。结果表明:林蛙卵油中含有18种脂肪酸,不饱和脂肪酸总含量为76.76%,多不饱和脂肪酸含量为40.25%;多不饱和脂肪酸富集工艺的最优条件:脲脂比为2、包合温度为-20℃、包合时间为15 h;林蛙卵油在-20℃、避光条件下保存,过氧化值变化最小。
In order to study the components of fatty acids,polyunsaturated fatty acid enrichment process and oxidation stability in the ovum oil of Rana dybowskii,fatty acids extracted from the ovum oil of Rana dybowskii were detected by GC-MS.Polyunsaturated fatty acids were separated and purified by urea package,and orthogonal test was performed.The ovum oil was stored in different conditions for 0,6,12,18,24 and 30 days.In the end,the oxidation stability was determined.Results showed that 18 kinds of fatty acids were separated from the ovum oil,the total content of unsaturated fatty acids was 76.76%,and the content of polyunsaturated fatty acids was 40.25%.The optimal conditions of polyunsaturated fatty acid enrichment process were urea-fat ratio of 2,inclusion temperature of-20℃and inclusion time of 15 h.When ovum oil was stored at-20℃in the dark,the minimal change of peroxide value was found.
作者
陈宁宁
常雷
岑欣桉
徐太成
尹云厚
卫功庆
CHEN Ningning;CHANG Lei;CEN Xin'an;XU Taicheng;YIN Yunhou;WEI Gongqing(College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130WS,China;Agricultural Station of Sandaohu Town of Jingyu County of Jilin Province,Jingyu 135202,China;College of Media,Guizhou Mirizu University,Guiyang 550025,China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2020年第1期96-102,共7页
Journal of Jilin Agricultural University
基金
吉林省科技发展计划项目(20130206027YY)。
关键词
东北林蛙
卵油
脂肪酸成分
气相色谱-质谱法
多不饱和脂肪酸
氧化稳定性
Rana dybowskii
ovum oil
fatty acid composition
gas chromatography-mass spectrometry(GC-MS)
polyunsaturated fatty acid
oxidation stability