双斑长跗萤叶甲成虫粪便活性成分鉴定

Identification of Active Components in Feces of Monolepta hieroglyphica(Motschulsky)

【目的】研究从双斑长跗萤叶甲Monolepta hieroglyphica(Motschulsky)成虫粪便挥发性化合物中,筛选对该叶甲具有电生理及行为活性的化合物。【方法】通过GC-MS采集鉴定双斑长跗萤叶甲粪便挥发性化合物的组分和含量,利用触角电位仪和“Y”型嗅觉仪测定该叶甲雌、雄成虫对其粪便中4种挥发物的电生理和行为反应。【结果】该叶甲成虫粪便挥发物中含有包括烯类、醛类、酯类、酸类、醇类等共29种化合物;其雌、雄成虫均对α-石竹烯、α-蒎烯、α-红没药醇的EAG反应较明显,且雌雄虫均对10μL/mL的α-红没药醇和α-蒎烯有较为明显的趋性。【结论】α-红没药醇和α-蒎烯可能为该叶甲成虫粪便中的有效活性成分。

【Objective】To screen out the compounds with electrophysiological and behavioral activities from the fecal volatile compounds of Monolepta hieroglyphica(Motschulsky)adults.【Method】The components and contents of the volatile compounds in the feces were collected and identified by using GC-MS.The electrophysiological and behavioral responses of the female and male adults of the beetle to four kinds of volatile compounds in the feces were measured by the EAG and"Y"olfactometer.【Result】There were 29 compounds in the feces of the adult beetle,including alkenes,aldehydes,esters,acids,alcohols,etc.The EAG responses of the female and male adults toα-caryophyllene,α-pinene,Dragosantol were obvious,and the male and female insects had obvious convergence to 10μL/mL Dragosantol andα-pinene.【Conclusion】Dragosantol andα-pinene may be the active components in the feces of the adult.