尿液外泌体circ_0040507联合PSA对前列腺癌诊断价值的初步研究

Preliminary study of diagnostic value of urinary exosomal circ_0040507 combined with PSA for prostate cancer

目的探讨尿液外泌体circ0040507及其联合前列腺特异性抗原(PSA)对前列腺癌的诊断价值。方法选择35例前列腺癌患者(前列腺癌组)和27例BPH患者(BPH组),采用第三代微滴数字PCR检测尿液外泌体circ0040507表达水平,以前列腺活组织检查(活检)病理结果为金标准,应用受试者工作特征(ROC)曲线分析尿液外泌体circ0040507、PSA及两者联合对前列腺癌的诊断效能。结果前列腺癌组和BPH组尿液外泌体circ0040507表达量分别为(8.35±5.87)copies/ml和(3.25±2.45)copies/ml,前列腺癌组尿液外泌体circ0040507表达量高于BPH组(P<0.001);尿液外泌体circ0040507、PSA及两者联合诊断前列腺癌的ROC曲线下面积分别为0.767(95%CI 0.649~0.896)、0.782(95%CI 0.668~0.896)、0.851(95%CI0.755~0.946)。结论尿液外泌体circ0040507联合PSA诊断前列腺癌具有较好的临床价值。

Objective To investigate the diagnostic value of urinary exosomal circ_0040507 combined with prostate-specific antigen(PSA)for prostate cancer.Methods Digital droplet polymerase chain reaction(ddPCR)was employed to detect the expression level of circ_0040507 in the urinary exosomes of 35 patients with prostate cancer and 27 patients with benign prostatic hyperplasia.The pathological results of prostate biopsy were regarded as the gold standard.The receiver operating characteristic(ROC)curve was delineated to analyze the sensitivity and specificity of urinary exosomal circ_0040507,PSA and the combination of them for the diagnosis of prostate cancer.Results The expression level of urinary exosomal circ_0040507 in the BPH group was(3.25±2.45)copies/ml,significantly lower compared with(8.35±5.87)copies/ml in the prostate cancer group(P<0.001).The area under ROC for circ_0040507,PSA,and the combination was 0.767(95%CI 0.649-0.896),0.782(95%CI 0.668-0.896)and 0.851(95%CI 0.755-0.946),respectively.Conclusion The determination of urinary exosomal circ_0040507 combined with PSA is a valuable biomarker for the diagnosis of prostate cancer.