碳量子点荧光猝灭法检测药物中的异鼠李素

Facile Synthesis of Fluorescence Carbon Quantum Dots and Their Application in Detection of Isorhamnetin in Medicines

以L-天冬氨酸为碳源,尿素为氮源,采用微波加热法制备荧光碳量子点(CDs),得到的CDs具有球形结构和单一分散性,平均粒径约为5 nm。红外光谱、X射线光电子能谱、紫外和荧光光谱的表征结果表明,合成的CDs具有较高的荧光稳定性,良好的水溶性和对异鼠李素(ISOR)高的选择性。在优化条件下,ISOR浓度在0.22~180 nmol/L范围内时与CDs荧光猝灭程度(IF0/IF)呈良好的线性关系,检出限(LOD)为1.32 nmol/L,回收率为90.8%~107%。结果表明,该CDs可用于ISOR的快速、高效、灵敏检测。

A kind of novel fluorescence carbon quantum dots(CDs)were fabricated by microwave heating method using L-aspartic acid and urea as the precursors.The obtained CDs exhibited good spherical shapes with an average size about 5 nm.In addition,the CDs were characterized by Fourier transform infared spectroscopy,X-ray photoelectron spectroscopy,ultraviolet-visible absorption spectroscopy and fluorescence spectroscopy.The CDs showed high fluorescence stability,good particle dispersion,excellent water solubility and remarkable selectivity toward isorhamnetin(ISOR).Under the optimized conditions,there was a good relationship between fluorescence quenched degree(IF0/IF)of the CDs and concentration of ISOR in the range of 0.22-180 nmol/L with a limit of detection(LOD)of 1.32 nmol/L.The CDs were successfully applied in the detection of ISOR in ginkgo biloba tablets with recoveries of 90.8%-107%.Results showed that the as-synthesized CDs could be used in ISOR detection with rapidness,efficiency and sensitivity.