展毛野牡丹酚类化学成分的研究

Phenolic constituents from Melastoma normale

为了明确展毛野牡丹的化学成分,该研究采用Diaion HP20SS、MCI gel、Sephadex LH-20柱层析和反相高效液相色谱等方法,对展毛野牡丹根和茎的醇提物分别进行了分离纯化。结果表明:从展毛野牡丹中分离得到11个化合物,它们的结构经波谱数据分析和鉴定。它们分别是4-羟基-3-甲氧基苯酚1-O-β-D-(6′-O-没食子酰)-吡喃葡萄糖苷(1)、3,4-二羟基苯乙醇4-O-β-D-(6′-O-没食子酰基)-吡喃葡萄糖苷(2)、龙胆酸5-O-β-D-(6′-O-没食子酰基)-吡喃葡萄糖苷(3)、2,4,6-三甲氧基苯酚1-O-β-D-(6′-O-没食子酰)-吡喃葡萄糖苷(4)、甲基6-O-没食子酰基-β-D-吡喃葡萄糖苷(5)、乙基6-O-没食子酰基-β-D-吡喃葡萄糖苷(6)、6′-O-没食子酰基黑樱苷(7)、没食子酸甲酯(8)、没食子酸乙酯(9)、2,6-二甲氧基对苯二酚4-O-β-D-吡喃葡萄糖苷(10)、2-甲氧基对苯二酚4-O-β-D-吡喃葡萄糖苷(11)。所有化合物均为首次从展毛野牡丹中分离得到,化合物2-7、10和11为首次从该属植物中分离得到。

To clarify the chemical constituents of Melastoma normale,the ethanol extracts of roots of and stems M.normale were respectively isolated and purified by various chromatographic methods such as Diaion HP20SS,MCI gel,Sephadex LH-20,and reversed-phase HPLC.Eleven compounds were obtained,and their structures were identified by spectral data analyses.They are 4-hydroxy-3-methoxyphenyl 1-O-β-D-(6′-O-galloyol)-glucopyranoside(1),3,4-dihydroxyphenethyol alcohol 4-O-β-D-(6′-O-galloyol)-glucopyranoside(2),gentisic acid 5-O-β-D-(6′-O-galloyl)-glucopyranoside(3),2,4,6-trimethoxyphenol 1-O-β-D-(6′-O-galloyl)-glucopyranoside(4),methyl 6-O-galloyl-β-D-glucopyranoside(5),ethyl 6-O-galloyl-β-D-glucopyranoside(6),6′-O-galloylprunasin(7),methyl gallate(8),ethyl gallate(9),2,6-dimethoxy-hydroquinone-4-O-β-D-glucopyranoside(10),and 2-methoxy-hydroquinone-4-O-β-D-glucopyranoside(11).All these compounds were obtained firstly from this plant,and compounds 2-7,10,and 11 were obtained from the genus Melastoma for the first time.