HPLC法测定不同产地和品种柠檬果皮中柠檬苦素和诺米林含量

Determination of limomin and nomilin of different varieties of lemon peel from different areas by HPLC

该研究建立了同时测定檬苦素和诺米林含量的高效液相色谱法,并采用该方法对8种不同柠檬样品中柠檬苦素和诺米林含量进行了测定。色谱条件如下:安捷伦Eclipse XDB-C18(250 mm×4.6 mm,5μm)色谱柱,流动相为0.1%磷酸水溶液(A)-乙腈(B),梯度洗脱,流速为1.0 mL·min^-1,柱温为30℃,波长为210 nm。结果表明:3个产地尤力克果皮中柠檬苦素含量依次为云南瑞丽(EUR_D)、四川安岳(EUR_C)和广东河源(EUR_Y),诺米林含量依次为EUR_D、EUR_C和EUR_Y;不同品种柠檬果皮中柠檬苦素含量依次为北京柠檬(M_NM)、粗柠檬(R_NM)、无核柠檬(S_NM)、热那亚(RNY)、里斯本(LSB),诺米林含量依次为S_NM、R_NM、LSB、RNY和M_NM。综上结果表明,不同产地和品种柠檬果皮中柠檬苦素和诺米林含量均存在不同程度差异,该HPLC法可为不同产地和不同品种柠檬果实质量鉴别、资源评价及深度开发提供理论依据。

This study established an high-performance liquid chromatography(HPLC)method for simultaneous determination of limonin and nomilin in Citrus limon,and the contents of limonin and nomilin in eight different lemon samples was determined by this method.The chromatographic condition was performed on an Agilent Eclipse XDB-C18(250 mm×4.6 mm,5μm)column.The mobile phase consisted of 0.1%aqueous phosphoric acid(A)and acetonitrile(B)was invoked as gradient elution with the flow rate at 1.0 mL·min^-1.The column temperature was set at 30℃and the detection wavelength was set at 210 nm.The results were as follows:The content of limonin in the lemon peel of three producing areas was Ruili,Yunnan(EUR_D),Anyue,Sichuan(EUR_C)and Heyuan,Guangdong(EUR_Y)respectively,while the content of nomilin was EUR_D,EUR_C and EUR_Y;The content of limonin in different varieties of lemon peel was Meyer lemon(M_NM),Rough lemon(R_NM),Seedless lemon(S_NM),Genoa(RNY)and Lisbon(LSB),while the content of nomilin were S_NM,R_NM,LSB,RNY and M_NM.The results displayed that the content of limonin and nomilin in lemon peel from different areas and varieties was different.The HPLC method can provide a theoretical basis for the quality identification,resource evaluation and in-depth development of lemon fruits from different areas and varieties.