8个地理种群红条毛肤石鳖COI基因的遗传多样性分析

Genetic Diversity Analysis of COI Gene in 8 Geographical Populations of Acanthochiton rubrolineatus

[目的]分析我国8个沿海潮间带红条毛肤石鳖地理种群的COI基因的遗传多样性。[方法]利用聚合酶链式反应(PCR)技术扩增8个地区80个红条毛肤石鳖样品的COI基因。使用DNASP5.10.01、MEGA7.0等软件对所得序列进行分析,得到片段碱基含量、遗传距离以及遗传多样性参数等数据,并通过构建系统发育树的方式,结合遗传多样性数据分析结果。[结果]获得的COI基因片段长度为659 bp,COI基因位于线粒体编码区中,平均A、T、C、G的碱基含量分别20.4%、41.7%、14.9%和23.0%,存在明显的AT偏向,结合Acanthochitona crinita为外群系统发育树的结果来看,中国沿海地区红条毛肤石鳖种群分成2个明显的聚群,将它们定义为北方群体和南方群体,遗传距离为0.00066~0.08785。单倍型遗传多样性数据显示,多样性数值较高,但核苷酸多样性较低。[结论]红条毛肤石鳖北方群体的遗传多样性与南方群体存在差异,2个群体应该分别采取相应措施来保护遗传多样性。

[Objective]To analyze the genetic diversity of COI gene in Acanthochiton rubrolineatus from 8 littoral geographical populations of China.[Method]COI gene of 80 samples of A.rubrolineatus from 8 geographical populations were amplified by polymerase chain reaction(PCR technology).And we used DNASP5.1001,MEGA7.0 and other softwares to analyze the sequences,calculate the data of fragment base content,distance and other genetic diversity parameters,and analyze the results by the means of constructing phylogenetic trees in combination of genetic diversity data.[Result]The length of COI gene fragment was 659 bp,COI gene was located in the encoding region of mitochondria,and the average content of bases A,T,G,C was 20.4%,41.7%,14.9%,23.0%respectively,showing a significant AT bias.Combined with the results of phylogenetic trees with the outgroup of Acanthochitona crinite,we found that all haplotypes of A.rubrolineatus populations in China coasts were clustered into two apparent geographic lineages in phylogenetic trees,named as Northern group and Southern group,the genetic distance ranged from 0.00066 to 0.08785.The results of haplotype genetic diversity reflected that Hd was high,but the nucleotide diversity was low.[Conclusion]There were obvious differences between the northern group and southern group of A.rubrolineatus in China,and the northern and southern group should be protected separately by taking some measures.