猫须草提取物体外抗肿瘤活性部位研究

Study on Anti-tumor Active Parts in vitro of the Extracts from Clerodendranthus spicatus

[目的]研究猫须草不同提取物对多种肿瘤细胞生长抑制的影响,筛选出猫须草的抗肿瘤活性部位。[方法]采用MTT法体外抗肿瘤活性试验研究猫须草提取物分别对人回盲肠癌细胞(HCT-8)、人肝癌细胞(BEL-7402)和人非小细胞肺癌细胞(A549)的生长抑制作用。[结果]猫须草乙醇提取物分别经石油醚萃取、乙酸乙酯萃取后对3种肿瘤细胞均具有良好的抑制活性,而正丁醇萃取物和萃取后的水层无抗肿瘤活性作用。[结论]该研究为后续开展的抗癌活性化学成分单体的提纯和体内抗肿瘤药理试验研究提供理论依据。

[Objective]The research aimed to study the different extracts of Clerodendranthus spicatus on growth inhibition of various tumor cells,and to screen out the anti-tumor active sites.[Method]MTT colourometry was used to determine the inhibitory effect of the different extracts of Clerodendranthus spicatus on hct-8,bel-7402 and A549 human tumor cells in vitro.[Result]The petroleum ether extract and ethyl acetate extract of spicatus had inhibitory activity on three kinds of human tumor cells,but the n-butanol extract and the water extract had no antitumor activity.[Conclusion]This research provides a theoretical basis for the subsequent purification of anti-cancer active chemical constituent monomers and in vivo anti-tumor pharmacological test research.