半夏自毒作用研究

Study on Autotoxicity of Pinellia ternate

将半夏叶和块茎水浸提液分别配制成不同浓度,采用生物测定方法,对半夏连作障碍的自毒作用进行初步研究。结果表明,低浓度的半夏叶和块茎水浸提液有促进种子萌发的作用,高浓度有抑制作用,同时,不同浓度叶和块茎水浸提液对半夏发芽率、发芽势及发芽指数的自化感作用均为负值,表现为抑制种子的萌发;当浓度为80 mg/mL时,叶和块茎水浸提液对种子萌发的自化感效应值最大,其中,叶水浸提液对发芽率、发芽势和发芽指数的自化感效应分别为-0.29、-0.27和-0.37,块茎水浸提液对发芽率、发芽势和发芽指数的自化感效应分别为-0.41、-0.24和-0.46。因此,半夏具有很强的自毒作用,而且自毒作用还存在部位差异,尤其以块茎水浸液的抑制作用最强,且随浸提液质量浓度的升高,化感作用加强。

The aqueous extracts of Pinellia ternate leaves and tubers were formulated to different concentrations,and the autotoxicity of Pinellia ternate continuous cropping obstacles was studied using bioassay methods.The results showed that the water extraction of leaf and tuber with the low concentration could promote seed germination and the high concentration inhibited seed germination.At the same time,the auto-allelopathy of Pinellia ternate germination rate,germination potential and germination index was negative with different concentrations of leaf and tuber water extracts,which showed the inhibition of seed germination.When the concentration was 80 mg/mL,the leaf and tuber water extracts had the largest allelopathy effect on seed germination.Among them,the self-sensitization effects of leaf water extract on germination rate,germination potential and germination index were-0.29,-0.27 and-0.37,respectively.The self-sensitization effects of tuber water extract on germination rate,germination potential and germination index were-0.41,-0.24 and-0.46,respectively.Therefore,Pinellia ternate has a strong autotoxic effect,and there are also differences in the location of the autotoxic effect,especially the tuber water extract has the strongest inhibitory effect,and the allelopathic effect increases with the increase of the mass concentration of the extract.