肝细胞癌亲环素J的表达及其作用

EXPRESSION AND ROLE OF CYCLOPHILIN J IN HEPATOCELLULAR CARCINOMA

目的探讨亲环素J(CYPJ)在肝细胞癌(HCC)组织中的表达及其作用。方法应用UALCAN和cBioPortal在线网站分析CYPJ在HCC中表达及其与临床病理特征关系,并通过实时荧光定量PCR(qPCR)检测40例HCC进行验证。分析CYPJ在HCC中mRNA表达水平,并通过Kaplan-Meier生存曲线评估病人预后。将CYPJ-RNAi克隆入慢病毒载体(LV),并构建阴性对照(NC-LV),分别转染SK-Hep1细胞,qPCR方法验证转染效率。用MTT法检测不同感染复数(MOI)对SK-Hep1细胞生长的影响。结果HCC组织中CYPJ mRNA表达高于癌旁正常组织(t=2.759,P<0.05),且与肿瘤病理分级有关(t=2.557,P<0.05)。Kaplan-Meier生存曲线分析显示,CYPJ mRNA高表达病人预后差(χ^2=9.464,P<0.01);通过沉默CYPJ基因表达可以抑制SK-Hep1细胞生长(t=3.231、8.976,P<0.01)。结论CYPJ mRNA高表达在HCC生长过程中具有重要作用,并影响病人预后。

Objective To investigate the expression and role of cyclophilin J(CYPJ)in hepatocellular carcinoma(HCC).Methods UALCAN and cBioPortal online websites were used to analyze the expression of CYPJ in HCC and its association with clinicopathological features,and the results were verified by quantitative real-time PCR analysis of 40 patients with HCC.The mRNA expression of CYPJ in HCC was analyzed and the Kaplan-Meier survival curve was used to evaluate patients’prognosis.CYPJ-RNAi was cloned into lentivirus vector(LV)and a negative control(NC-LV)was established,and then they were transfec-ted into SK-Hep1 cells,respectively.The transfection efficiency was verified by qPCR.MTT assay was used to observe the effect of multiplicity of infection(MOI)on the growth of SK-Hep1 cells.Results The expression of CYPJ in HCC tissue was significantly higher than that in adjacent normal tissue(t=2.759,P<0.05),which was associated with the pathological grade of tumor(t=2.557,P<0.05).The Kaplan-Meier survival curve analysis showed that the patients with high expression of CYPJ tended to have poor prognosis(χ^2=9.464,P<0.01).Silencing of the CYPJ gene inhibited the growth of SK-Hep1 cells(t=3.231,8.976;P<0.01).Conclusion High expression of CYPJ plays an important role in HCC tumor growth and affects patients’prognosis.