杜洛克猪HLCS基因多态位点与生长发育性状的相关性分析

Association Analysis on HLCS Gene Polymorphic Site with Growth and Development Traits in Duroc Pigs

本研究旨在明确羧化全酶合成酶(holocarboxylase synthetase,HLCS)基因突变与杜洛克猪生长发育性状的相关性。利用Illumina SNP60芯片测序结果研究HLCS基因的突变位点,分析突变位点的多态性信息、突变位点与生长发育性状的关联性及突变位点的单倍型。结果显示,杜洛克猪HLCS基因具有4个SNPs位点:Ssc13:200455646 T>C、Ssc13:200458655 G>C、Ssc13:200504530 G>T、Ssc13:200551864 T>G,均符合Hardy-Weinberg平衡状态(P>0.05),呈现中度多态性。关联分析结果发现,Ssc13:200455646 T>C位点TT基因型100 kg背膘、剩余采食量均显著高于TC和CC基因型(P<0.05);Ssc13:200458655 G>C位点GG基因型个体100 kg背膘、剩余采食量均显著高于CG和CC基因型(P<0.05);Ssc13:200504530 G>T位点GT基因型平均日采食量、平均日增重均显著高于TT基因型(P<0.05),GT基因型个体100 kg日龄显著小于TT基因型(P<0.05);Ssc13:200551864 T>G位点TG基因型平均日采食量、平均日增重均显著高于GG基因型(P<0.05),TG基因型个体100 kg日龄显著小于GG基因型(P<0.05);其余性状各位点不同基因型间无显著差异(P>0.05)。Ssc13:200455646 T>C和Ssc13:200458655 G>C位点呈现高度连锁,Ssc13:200504530 G>T与Ssc13:200551864 T>G位点呈现高度连锁。结果表明,HLCS基因4个SNPs位点的多态性与杜洛克猪的剩余采食量、平均日采食量、平均日增重、100 kg日龄和100 kg背膘均具有显著相关性,可为猪的生长发育遗传改良奠定基础。

The objective of this study was to clarify the correlation between SNP of holocarboxylase synthetase(HLCS)gene and growth and development traits in Duroc pigs.SNP of HLCS gene was obtained using the sequencing results of Illumina SNP60 chip,the polymorphism information of SNP,the correlation between SNP of HLCS gene and growth and development traits,and the haplotype of SNP were analyzed in this study.The results indicated that 4 SNPs(Ssc13:200455646 T>C,Ssc13:200458655 G>C,Ssc13:200504530 G>T and Ssc13:200551864 T>G)of HLCS gene were detected in Duroc pigs,which were in Hardy-Weinberg equilibrium(P>0.05),showing moderate polymorphism.The correlation analysis results showed that the 100 kg back fat(100 kg BF)and remaining food intake(RFI)of TT genotype in Ssc13:200455646 T>C were significantly higher than that of TC and CC genotypes(P<0.05).The 100 kg BF and RFI of GG genotype in Ssc13:200458655 G>C were significantly higher than that of CG and CC genotypes(P<0.05).The average daily feed intake(ADFI)and average daily gain(ADG)of GT genotype in Ssc13:200504530 G>T were significantly higher than that of TT genotype(P<0.05),the 100 kg age of GT genotype in Ssc13:200504530 G>T were significantly lower than that of TT genotype(P<0.05).The ADFI and ADG of TG genotype in Ssc13:200551864 T>G were significantly higher than that of GG genotype(P<0.05),the 100 kg age of TG genotype in Ssc13:200551864 T>G were significantly lower than that of GG genotype(P<0.05).There was no significant difference of other traits in different genotypes of SNPs(P>0.05).It showed high linkage of Ssc13:200455646 T>C and Ssc13:200458655 G>C,Ssc13:200504530 G>T and Ssc13:200551864 T>G,respectively.The results indicated that there were significant correlation between 4 SNPs of HLCS gene and RFI,ADFI,ADG,100 kg age and 100 kg BF in Duroc pigs,it could lay a foundation for the genetic improvement of growth and development in pigs.