安卡拉病毒贵州流行株penton基因分子特性分析

Molecular Characteristics Analysis of penton Gene of Ankara Virus Guizhou Strains

为了解安卡拉病毒贵州流行株penton基因分子特征,本试验参考GenBank上FAdV基因序列设计合成特异性引物,对penton基因CDS区进行扩增,运用生物信息学软件对penton基因的核苷酸序列、蛋白质理化性质、信号肽、二级结构、三级结构和B细胞表位进行分析。结果显示,3株FAdV(GZ-BJ、GZ-QL、GZ-LPS)penton基因片段大小均为1578 bp。系统进化树分析显示,GZ-BJ、GZ-QL、GZ-LPS均属于FAdV-4型,3株FAdV-4核苷酸同源性在99.9%~100%之间,推导氨基酸序列同源性在99.8%~100%之间;与国内其他分离株、国外分离株差异不大。蛋白理化性质分析显示,该蛋白属于不稳定的、不具有跨膜结构的亲水蛋白,其二级结构主要以无规则卷曲为主(54.29%);结构域预测结果显示,penton蛋白只含有一个低复杂度区域;B细胞表位预测发现,penton基因编码的氨基酸可能存在3个优势抗原表位区,分别为5-171、207-236和420-462位氨基酸处。本试验结果表明,安卡拉病毒penton基因相对保守,存在多个优势抗原表位,可作为安卡拉病毒防控研究靶蛋白,为该病毒防控与致病机理研究奠定基础。

To understand the molecular characteristics of penton gene of Ankara virus Guizhou strains,specific primer was designed and synthesized based on the sequence of FAdV gene,and the penton gene CDS regions were amplified.The nucleotide sequence,physical and chemical properties of protein,signal peptide,secondary structure,tertiary structure and B cell epitope of penton gene were analyzed by bioinformatics software.The results showed that three FAdV(GZ-BJ,GZ-QL and GZ-LPS)penton gene fragments were 1578 bp.Phylogenetic tree analysis showed that GZ-BJ,GZ-QL and GZ-LPS all belonged to FAdV-4 type.The nucleotide homology of three FAdV-4 strains ranged from 99.9%to 100%,and the amino acid sequence homology ranged from 99.8%to 100%.There was a little difference with other domestic isolates and foreign isolates.The physical and chemical properties of the protein showed that the protein was unstable and had no transmembrane structure.The secondary structure was mainly composed of random coil(54.29%).The domain prediction results showed that the penton protein contained only a low complexity region.B cell epitope prediction showed that there were three dominant epitopes in the amino acid encoded by penton gene:5-171,207-236 and 420-462.The results indicated that the Ankara virus penton gene was relatively conserved and there were multiple dominant epitopes.It could be used as a target protein for Ankara virus prevention and control research,laying a foundation for the virus prevention and control and pathogenic mechanism research.