蜱传脑炎病毒感染小鼠脑组织单核细胞趋化蛋白1(MCP-1)、CD68和核因子κB(NF-κB)表达增加

Tick-borne encephalitis virus(TBEV)enhances the expression of MCP-1,CD68 and NF-κB in infected mouse brain tissue

目的探讨蜱传脑炎病毒(TBEV)感染小鼠单核细胞趋化蛋白1(MCP-1)、CD68和核因子κB(NF-κB)表达情况。方法1周龄BALB/c小鼠8只,随机分为对照组和感染组,分别进行PBS或TBEV颅内注射。感染8 d后处死取一半脑组织固定,HE染色检测脑组织病理改变;免疫组织化学染色法检测小鼠大脑MCP-1、NF-κB和CD68的表达,镜下观察并统计阳性细胞数量;免疫荧光化学染色法结合激光扫描共聚焦显微镜观察MCP-1和CD68的共表达情况。结果与对照组相比,TBEV感染后小鼠脑组织MCP-1、NF-κB和CD68阳性细胞数均显著增加,MCP-1在CD68阳性及非阳性细胞中均有表达。结论TBEV感染小鼠CD68、NF-κB、MCP-1表达增强。

Objective To investigate the expression of monocyte chemoattractant protein 1(MCP-1),CD68 and nuclear factor kappa B(NF-κB)in mice infected by tick-borne encephalitis virus(TBEV).Methods One-week-old BALB/c mice were randomly divided into control group and infection group with 4 mice in each group.PBS and TBEV was intracranially injected to the control and infection groups,respectively.The mice were sacrificed and half of the brain in each group was fixed after 8 days of infection.HE staining was performed to observe the pathological changes.The expression of MCP-1,NF-κB and CD68 in the brain tissue were detected by immunohistochemical staining and the number of positive cells was counted under a microscope.Immunofluorescence technique combined with laser scanning confocal microscope was used to detect the co-expression of MCP-1 and CD68.Results Compared with the control group,the number of positive cells that expressed MCP-1,NF-κB and CD68 significantly increased in the TBEV infection group and MCP-1 was expressed in both CD68-positive and non-CD68-positive cells.Conclusion TBEV can enhance the expression of MCP-1,CD68 and NF-κB in mouse brain tissue.