PTEN与Cyclin G1蛋白在婴幼儿血管瘤组织中的表达及意义

Expressions and Significance of PTEN and Cyclin G1 Proteins in Hemangioma Tissues of Infants

目的:探究血管瘤组织第10号染色体丢失的磷酸酶及张力蛋白同源物(PTEN)与细胞周期素G1(Cyclin G1)的表达。方法:回顾性分析142例血管瘤患儿临床资料,比较血管瘤瘤体组织与瘤旁组织、增殖期与消退期血管瘤组织、术后6个月内复发与未复发血管瘤组织PTEN、Cyclin G1蛋白表达情况,以受试者工作特征(ROC)曲线评估上述指标对血管瘤复发的预测效能,通过Pearson相关系数模型分析上述指标与血管瘤表面积、深度、微血管密度(MVD)的相关性。结果:PTEN蛋白表达情况比较显示,血管瘤瘤体组织明显低于瘤旁组织,增殖期血管瘤组织明显低于消退期血管瘤组织,预后复发血管瘤组织明显低于预后未复发血管瘤组织(P<0.05);Cyclin G1蛋白表达情况比较显示,血管瘤瘤体组织明显高于瘤旁组织,增殖期血管瘤组织明显高于消退期血管瘤组织,预后复发血管瘤组织明显高于预后未复发血管瘤组织(P<0.05)。且PTEN、Cyclin G1蛋白表达水平联合预测复发的灵敏度与特异度可达64.10%、78.64%。血管瘤组织PTEN蛋白表达与病灶表面积、深度、MVD均呈显著负相关性(r<0,P<0.05),Cyclin G1蛋白表达与病灶表面积、深度、MVD均呈显著正相关性(r>0,P<0.05)。结论:血管瘤组织存在PTEN蛋白低表达与Cyclin G1蛋白过表达情况,且表达水平与病情、预后均联系紧密,可为疾病发生、发展的机制研究及临床预后评估提供指导。

Objective To explore the significance of phosphatase and tension homology deleted on chromosome ten(PTEN)and cyclin G1(Cyclin G1)in hemangioma tissues.Methods The clinical data of 142 children patients with hemangiomas were retrospectively analyzed.The protein expressions of PTEN and Cyclin G1 were compared between hemangioma tissues and para-tumor tissues,between proliferative and regressive hemangioma tissues and between recurrent and non-recurrent hemangiomas within 6 months after surgery.The receiver operating characteristic(ROC)curve was used to evaluate the predictive efficacy of the above indexes on recurrence of hemangiomas.The correlation between the above indexes and the surface area,depth and microvessel density(MVD)of hemangiomas was analyzed by Pearson correlation coefficient model.Results The expression of PTEN protein in hemangioma tissues was significantly lower than that in para-tumor tissues,and the expression in proliferative hemangioma tissues was significantly lower than that in regressive hemangioma tissues,and the expression in tissues with prognosis of recurrent hemangioma was significantly lower than that in tissues with prognosis of non-recurrence(P<0.05).The expression of Cyclin G1 protein in hemangioma tissues was significantly higher than that in para-tumor tissues,and the expression in proliferative hemangioma tissues was significantly higher than that in regressive hemangioma tissues,and the expression in tissues with prognosis of recurrent hemangioma was significantly higher than that in tissues with prognosis of non-recurrence(P<0.05).The sensitivity and specificity of combined detection of PTEN and Cyclin G1 proteins were 64.10%and 78.64%in predicting recurrence.The PTEN protein expression in hemangioma tissues was significantly negatively correlated with surface area,depth and MVD of lesions(r<0,P<0.05),and the Cyclin G1 protein expression was significantly positively correlated with surface area,depth and MVD of lesions(r>0,P<0.05).Conclusion There is a low expression of PTEN protein and overexpression of Cyclin G1 protein in hemangioma tissues,and the expression levels are closely related to the disease condition and prognosis.They can provide guidance for the mechanism study of disease occurrence and development and the evaluation of clinical prognosis.