雷公藤多苷抑制睾丸支持细胞增殖及其相关机制研究

Study on the Mechanism of Tripterygium Wilfordii Glycosides Inhibiting the Proliferation of Sertoli Cells

目的探索雷公藤多苷(GTW)对睾丸支持细胞增殖的影响机制。方法制备GTW含药血清,与体外培养的睾丸支持细胞共培养,以观察GTW对睾丸支持细胞增殖的影响机制,同期与无药血清组、补肾中药组作对照;用MTT法测细胞增殖率,用流式细胞仪测细胞周期,用Western blot检测Caspase-9、Caspase-3蛋白表达。结果 (1)与无药血清组相比,GTW组睾丸支持细胞增殖率降低(P均<0.05),补肾中药组细胞增殖率变化差异无统计学意义(P>0.05);(2)细胞周期:与无药血清组相比,GTW组在G1期中细胞占比增多,S期细胞占比减少(P均<0.05);(3)与无药血清组相比,GTW组Caspase-9、Caspase-3蛋白表达增高(P均<0.05)。结论 GTW抑制睾丸支持细胞增殖,可能与细胞凋亡有关。

Objective To explore the mechanism of tripterygium wilfordii glycoside(GTW)on the proliferation of sertoli cells(SC).Methods The serum containing GTW was prepared and co-cultured with the SC in vitro to observe the effect mechanism of GTW on the proliferation of SC.At the same time,it was compared with the serum-free group,and the kidney tonifying Chinese medicine group.The cell proliferation rate was measured by MTT method,the cell cycle was measured by flow cytometry,and the expression of Caspase-9 and Caspase-3 protein was detected by Western blot.Results(1)Compared with the serum-free group,the proliferation rate of SC in GTW group decreased(P all<0.05),and there was no significance in the kidney tonifying Chinese medicine group(P>0.05).(2)Compared with the serum-free group,the percentage of cells in GTW group increased in G1 phase,while that in S phase decreased(P all<0.05).(3)Compared with the serum-free group,the expression of Caspase-9 and Caspase-3 protein in GTW group was higher(P all<0.05).Conclusion GTW can inhibit the proliferation of SC,it may be related to apoptosis and kidney tonifying herbs can antagonize the damage.