老黄酶OYE家族的蛋白质工程的研究进展

Advances in Protein Engineering of the Old Yellow Enzyme OYE Family

老黄酶OYE家族酶是一类广泛分布的能够催化烯烃化合物不对称还原的酶类,其能够用于多种手性化合物的制备。分析了OYE家族酶的系统分类及催化反应类型,针对目前该类酶在应用过程中出现的稳定性差、活性低及底物特异性强等问题,综述了蛋白质工程方法对该类酶进行改造的研究进展,为深入研究该家族酶的催化机制及进一步改造提供参考,同时为进一步拓展OYE酶的工业化应用奠定基础。

The old yellow enzyme family( OYEs) is a large family which widely distributed and capable of catalyzing the asymmetric reduction of olefin compounds. It can be used in the preparation of various chiral compounds. The system classification and catalytic reaction types of OYEs were analyzed. Meanwhile,protein engineering methods on the modification of the stability,activity and switching the substrate specificity of OYEs are also discussed. Some clues for further study the catalytic mechanism of OYEs and lays the foundation for further expanding the industrial application of OYEs were given.