细粒棘球绦虫Eg10诱导树突状细胞产生Th2免疫反应

Antigen 10 of Echinococcus granulosus(Eg10)induce DC production Th2 response

目的研究细粒棘球绦虫蛋白10(antigen 10 of Echinococcus granulosus Eg10)对小鼠感染细粒棘球绦虫免疫机制的影响。方法通过体外培养骨髓来源的小鼠树突状细胞(Bone marrow dendritic cell,BMDC),用不同的抗原包囊囊液(hydatid fluid HF)、Eg10、脂多糖(lipopolysaccharide LPS)单独或联合作用DC,共6组。通过扫描电镜和透射电镜观察各组DC形态学的变化;通过流式细胞仪检测DC的吞噬能力、迁移能力的变化、刺激T细胞增殖能力的变化及DC表面分子的表达情况;利用ELISA检测DC分泌各种细胞因子的变化。结果Eg10抑制DC向成熟状态发展,与囊液对照组相比,DC表面仅增多了褶皱,两者均可提高DC吞噬能力;刺激T细胞的增殖能力很弱,但是能够提高Treg细胞的含量;同时发现Eg10组DC表面分子MHCII和CD86的表达量高于空白组,分泌高含量的IL-10,IL-6。通过给予LPS联合刺激DC形态趋于半成熟状态,细胞表面树突增加,表面分子MHCII、CD80、CD40的表达增高,CD86的表达量降低;IL-10和IL-6的含量也相应的下降。结论Eg10诱导DC产生Th2反应。

To explore the immune mechanism of the mice immunized with Eg10 were infected with Echinococcus granulosus more severe than control group.The bone marrow dendritic cell(BMDC)of mice were obtained and cultured with different antigens including Eg10,hydatid fluid(HF)and lipopolysaccharide(LPS).The morphology of DCs in different groups were observed by electronic microscope,the capacity of up-taking,migrating,surface molecular and T cell proliferation capability were detected with flow cytometry.cytokines(IL-10,IL-6,IL-12p70 and TNF-α)in DCs supernatant were detected by Enzyme-linked immunosorbent assay(ELISA).Results showed that Eg10 like HF inhibited the DCs maturation,and the folds on superficial DCs of the two groups became more than control group and had very weak capability induce T cell proliferation,but T regulation cells increased.Eg10 group’s DCs had higher up-taking ability and expressed lower surface molecular of DCs,only MHCII and CD86 were higher than control group significantly(P<0.05);and secreted cytokines Interleukin-10(IL-10)and IL-6 higher level than other groups.However,after added LPS DCs became semi-mature status and more dendrites grow on surface,and surface molecular MHCII、CD80、CD40 expressed higher than other groups except for LPS group,but CD86 level were decreased.Meanwhile,the level of IL-10 and IL-6 were also decreased.So,these data indicated that Eg10 inhibited DC maturation and induced DCs produce Th2 immune response.