摘要
为优化微藻-细菌共生体系对畜禽养殖废水中碳氮磷去除的参数条件,利用响应面分析法(Response surface methodology,RSM)中的Box-Behnken中心组合设计(BBC),以接种比例、曝气量以及初始氨氮浓度为试验变量,以污染物去除率为响应值开展试验。响应面分析结果表明,对于COD去除的最佳条件为:活性污泥与微藻接种比例为6.0(m/m)、曝气量2.0 L·min^-1、初始氨氮浓度750 mg·L^-1,此时COD去除率达92%以上。对于总氮(Total nitrogen,TN)的去除,当接种比例5.0(m/m)、曝气量1.5 L·min^-1、初始氨氮浓度750 mg·L^-1时,其去除率可达最大值(53%)。而对于磷酸盐的去除,当接种比例6.0(m/m)、曝气量1.5 L·min^-1、初始氨氮浓度600 mg·L^-1时,试验前96 h内便可达到100%的去除率。进一步对生物量检测发现,初始条件分别为曝气量1.5 L·min^-1、初始氨氮浓度900 mg·L^-1、接种比例4.0(m/m)或曝气量1.0 L·min^-1、初始氨氮浓度750 mg·L^-1、接种比例4.0(m/m)时,微藻生物量产量最高,可达到1.63~1.64 g·L^-1。研究表明,通过响应面法可以优化藻菌共生体系对畜禽养殖废水的处理工艺。对于不同的目标污染物,具有不同的最优参数组合。综合考虑各因素对各目标污染物去除效果的影响,可以选择废水处理工艺最优参数组合。通过回收在废水处理过程中生长的藻菌共生体用于后续生物质利用,可实现良好的经济价值,提高该工艺在污水深度处理中的应用前景。
In order to achieve the optimized parameters for nutrient removal from livestock wastewater using the microalgae-bacteria symbiosis system,the Box-Behnken central combination design(BBC)of response surface methodology(RSM)was used.The selected experimental variables were inoculation proportion(IP),aeration rate(AR),and initial ammonium nitrogen(NH+4-N)concentration and the response value was pollutant removal efficiency(RE).The results of RSM showed that the optimal conditions for chemical oxygen demand(COD)removal were:activated sludge to microalgae IP at 6.0(m/m),AR at 2.0 L·min^-1 and initial NH+4-N concentration of 750 mg·L^-1with the highest COD RE over 92%.For total nitrogen(TN)removal,the best RE of 53%was achieved when IP was 5.0(m/m),AR was 1.5 L·min^-1,and initial NH+4-N concentration was 750 mg·L^-1.For phosphate removal,the RE reached 100%in 96 h with the IP at 6.0,the AR at 1.5 L·min^-1,and the initial NH+4-N concentration of 600 mg·L^-1.Further testing on biomass production showed that the highest yield of microalgae(1.63~1.64 g·L^-1)was achieved with AR at 1.5 L·min^-1,initial NH+4-N concentration of 900 mg·L^-1,and IP at 4.0(m/m)or AR at 1.0 L·min^-1,initial NH+4-N concentration of 750 mg·L^-1,and IP at 4.0(m/m).The results showed that RSM could be utilized for the optimization of livestock wastewater treatment by the microalgae-bacteria symbiosis system.There were different optimal parameter combinations for different targeted pollutants.Considering all parameters that influence the pollutant REs,the optimized parameter combination can be selected.The microalgae-bacteria consortia harvested from the wastewater treatment process can be further utilized as biomass resources,which will bring good economic value and further raise the application prospects of the process.
作者
曾慧卿
刘强
肖丛亮
郭远涛
孙盛进
辛佳期
李昆
ZENG Hui-qing;LIU Qiang;XIAO Cong-liang;GUO Yuan-tao;SUN Sheng-jin;XIN Jia-qi;LI Kun(School of Resource Environment and Chemical Engineering,Nanchang University,Key Laboratory of Poyang Lake Environment and Resource Utilization,Ministry of Education,Nanchang 330038,China)
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2020年第6期1368-1379,共12页
Journal of Agro-Environment Science
基金
国家自然科学基金项目(51768043)
中国博士后科学基金项目(2017M622106)
江西省青年科学基金项目(20171BAB216037)
江西省教育厅科研基金项目(GJJ160214)。
关键词
污水深度处理
畜禽养殖废水
藻菌共生
营养盐回收
响应面法
advanced wastewater treatment
livestock wastewater
microalgae-bacteria symbiosis
nutrient salts recovery
response surface methodology