二维层状钴铁双氢氧化物用于癌症协同光治疗

Application of Fe-Co Two-Dimension-Layered Double Hydroxide in Synergetic Therapy of Cancer

癌症的光子疗法是一种选择性治疗新技术,近几年得到迅猛的发展,该疗法拥有创伤小、选择性好且毒性低、无耐药性等优点。我们采用水热合成法制备了一种新型的层状钴铁双氢氧化物纳米片(Co-Fe-LDH),利用其具有较大比表面积、稳定高、生物相容性好等的特点,负载光敏剂IR783(LDH-IR783),在近红外激光的刺激下实现癌症的光热光动力协同光治疗。对所合成的Co-Fe-LDH进行了组成、形貌、光学性质、活性氧(ROS)生成、热量释放等表征,并在细胞及活体水平进行抗癌测试。实验结果表明,所制备的纳米复合物具有稳定的结构、高IR783负载率以及良好的分散性,并且,在近红外光源照射下表现出优异的光热/光动力效应,能够快速产生大量的活性氧,迅速地释放热量,产生显著的光毒性,能够有效地诱导HeLa细胞的凋亡。体内抗癌实验表明,所制备的纳米复合物有效地抑制了实体肿瘤的生长,并且未对正常组织产生明显的损伤,毒副作用较低。这些初步结果将为光热/光动力协同纳米粒子的设计和应用提供新的思路。

Photon therapy is a new selective cancer treatment technology which has been developed rapidly in recent years.It has the advantages of small trauma,good selectivity,low toxicity and no drug resistance.In this paper,we used hydrothermal synthesis method to prepare a new kind of layered Co-Fe double hydroxide nanosheet(Co-Fe-LDH)which has the characteristics of large specific surface area,high stability and good biocompatibility and is used to load photosensitizer IR783(LDH-IR783)to realize the photothermal/photodynamic cooperative phototherapy of cancer under the stimulation of near-infrared laser.We characterized the composition,morphology,optical property,reactive oxygen species(ROS)generation and heat release of LDH-IR783 and tested its anticancer activity at the cellular and in vivo levels.The results indicate that the nanocomposite has a stable structure,high IR783 loading efficiency and good dispersibility,exhibits excellent photothermal/photodynamic effects under near-infrared light irradiation through producing considerable ROS and releasing heat rapidly to induce severe phototoxicity to cancer cells.Both in vitro and in vivo experiments display that the nanocomposite can effectively induce apoptosis of HeLa cells and significantly inhibit the growth of solid tumors without obvious side effects and damage to normal tissue.The preliminary results will provide new ideas for the design and application of photo-thermal/photodynamic synergistic drugs.