长期鼻腔冲洗对正常鼻黏膜免疫屏障的影响

Effect of long-term nasal irrigation on the immune barrier of normal nasal mucosa

目的探讨长期鼻腔冲洗是否会对正常鼻黏膜的免疫屏障功能造成影响。方法55例健康志愿者随机分为两组:生理盐水冲洗(NSI)组29例,男13例,女16例;年龄18~61岁,平均年龄37.90岁。高渗盐水冲洗(HSI)组26例,男13例,女13例;年龄18~66岁,平均年龄40.65岁。NSI组以0.9%的温盐水(40°C)进行鼻腔冲洗,HSI组以3%的温盐水(40°C)进行鼻腔冲洗;两组均为每日1次,晚睡前30 min进行冲洗,干预时间为3个月。干预前和干预3个月后时分别获取两组受试者的鼻腔冲洗液(NLF),并对NLF中溶菌酶(LYSO)、乳铁蛋白(LF)、表面活性蛋白A(SPA),分泌型免疫球蛋白A(SIgA)的浓度进行检测。结果两组受试者干预前性别、年龄及NLF中LYSO、LF、SPA和SIgA的浓度之间差异无统计学意义(P>0.05),干预后NSI组的NLF中4种检测指标与干预前比较其浓度差异无统计学意义(P>0.05),干预后HSI组的NLF中4种检测指标与干预前比较其浓度差异无统计学意义(P>0.05),干预后两组受试者的NLF中4种检测指标的含量差异无统计学意义(P>0.05)。结论长期鼻腔冲洗对正常鼻黏膜的免疫屏障没有不利影响。

Objective To investigate the impact of long-term nasal irrigation(NI)on the function of immunological defense in normal nasal mucosa.Methods 55 healthy volunteers completed the study.They were randomly divided into 2 groups,normal saline irrigation(NSI)group and hypertonic saline irrigation(HSI)group.The NSI group mean included 29 volunteers(male 13,female 16),age ranged from 18 to 61 years old with an average of 37.90,and those of the HIS group were 26(male 13,female 13)with an average of 40.65(ranged from 18 to 66).Patients of the NSI group received NI using 0.9%saline and those of the HSI group with 3%saline with a temperature of 40°C,once a day,half an hour before sleeping in the evening.The total period of intervention was 3 months(90 days).The concentrations of lysozyme(LYSO),lactoferrin(LF),surfactant protein(SP)A and secretory immunoglobulin(SIg)A in nasal lavage fluid(NLF)from the two groups were examined before and after intervention of NI.Results There were no statistical differences between NSI and HSI groups in sex,age,and contents of LYSO,LF,SPA and SIgA in NLF before NI(P>0.05).The concentrations of these four proteins were not changed significantly after NSI and HSI treatment(P>0.05).In addition,there were no significant differences in these substance expressions in NLF between these two groups after the NI intervention(P>0.05).Conclusion Long-term NI treatment may not have harmful effect on the immune barrier of normal nasal mucosa.