无患子果皮提取物化学成分及其对白色念珠菌的抑制作用

Chemical Component and Inhibition Activity Against Candida albicans of Sapindus mukorossi Peel Extract

采用水提发酵和醇提法提取无患子果皮,得到2种提取物,通过HPLC-DAD/MS分析提取物的化学成分,并采用最小抑菌浓度(MIC)和最低杀菌浓度(MBC)比较2种提取物对白色念珠菌的体外抑制作用。结果表明:无患子水提发酵物(WFE)中总皂苷纯度为80.23%,高于醇提物(EE)的纯度75.50%,2种提取方法共得到13种皂苷,包括6种倍半萜皂苷和7种三萜皂苷,其中WFE和EE各12种皂苷,三萜皂苷分别占66.07%和80.39%;体外抑菌研究发现:WFE和EE均具有显著的抑菌活性,其MIC值分别为62.50和31.25 mg/L,MBC值分别为250和125 mg/L。通过外阴阴道念珠菌模型(VVC)考察2种提取物的体内治疗效果,发现WFE能显著降低(P<0.05)小鼠阴道内的真菌载量和炎症因子(IL-6,IL-8),2种提取物均能减轻阴道组织损伤程度,且WFE治疗效果更好。

The pericarp of Sapindus mukorossi was extracted by water fermentation extract and ethanol extract and two kinds of extracts were obtained,with the comparative analysis of the chemical composition of two methods.The results showed that the purity of total saponins in water fermentation extract(WFE)(80.23%)was higher than that of ethanol extract(EE)(75.50%).Based on HPLC-DAD/MS,a total of 13 saponins were identified including 6 kinds of sesquiterpene glycosides and 7 kinds of triterpenoid saponins.WFE and EE both contained 12 saponins,of which triterpenoid saponins accounted for 66.07%and 80.39%,respectively.The MIC and MBC were used to compare in vitro antifungal activities.It was found that the WFE and EE all had significant antifungal activities,the MIC values were 62.50 and 31.25 mg/L and the MBC values were 250 and 125 mg/L,respectively.A mouse model of vulvovaginal candidiasis(VVC)was established to examine in vivo inhibitory effects.Compared with the negative group,the WFE significantly reduced(P<0.05)the fungal burden and inflammatory factor(IL-6,IL-8).Both WFE and EE could improve VVC histopathology(P<0.05).Therefore,S.mukorossi pericarp extracts had antifungal activity against Candida albicans in vitro and in vivo,and the treatment effect of the WFE was better.