磷脂酰肌醇激酶-3、蛋白激酶在绝经后髋部骨折患者骨骼肌组织中的表达及其临床意义

Expression and Significance of Phosphatidylinositol Kinase-3 and Protein Kinase Akt in Mkele-tal Muscle of Postmenopausal Hip Fracture Patients Mieralimu

[目的]探讨磷脂酰肌醇激酶-3(PI3K)、蛋白激酶(Akt)在绝经后髋部骨折患者骨骼肌组织中的表达及其临床意义.[方法]前瞻性选取2017年1月至2019年6月在本院骨科接受手术治疗的绝经后髋部骨折患者80例,根据患者双能量X线骨密度(DXA)水平分为骨量正常组(-1≤T≤1,A组)、骨量减少组(-2.50.05).Akt、PI3K蛋白和mRNA表达水平均与患者骨密度值均呈显著正相关(P<0.05).[结论]绝经后骨质疏松患者骨骼肌中PI3K/Akt信号通路被激活,Akt、PI3K蛋白和mRNA表达水平均增高,且与骨质疏松程度呈正相关,推测PI3K/Akt对骨骼-肌肉形成和重建的起重要调控作用.

[Objective]To investigate the expression levels and elinical signifiance of phosphatidylinositol kinase 3(PI3K)and protein kinase(Akt)in skeletal muscle of postmenopausal hip fracture patients.[Methods]Prospectively selected a total of 80 post-menopausal patients who underwent surgery for hip fracture in our department from Jan-uary 2017 to June 2019.Patients were divided into 4 different groups according to the dual-energy X-ray bone density(DXA)levels.They were normal bone mass group(-1≤T≤1,group A),bone mass reduction group(-2.5<T<-1,group B),osteoporosis Group(T≤-2.5,group C)and severe osteoporosis group(T≤-2.5 with one or more brittle fractures,group D).A small amount of muscle tissue was taken from the lateral femoral muscle during the operation for RNA extract,quantitative Real-time PCR(RT-PCR)was detected for mRNA expression level of phosphatidyl inositol kinase-3(PI3K)and protein kinase(Akt),while the protein expression levels of PI3K and Akt were measured by immunohistochemical technique.The correlation of PI3K and Akt with the bone density was ana-lyzed as well.[Results]Both mRNA and protein expression levels of Akt and PI3K in the group D were higher than those in other groups(P<0.05).The mRNA and protein expression levels of Akt and PI3K in the group C was higher than that that in the group B(P<0.05).However,the mRNA and protein expression levels of Akt and PI3K were not statistically significant difference between the group A and B(P>0.05).The expression levels of AKT and PI3K were positively correlated with the bone mineral density of the patients.[Conclusion]The PI3K/Akt signaling pathway is activated in skeletal muscle of postmenopausal osteoporosis patients,and the mRNA and protein expression levels of PI3K and Akt are increased and also positively correlated with the degree of osteoporosis.It suggests that PI3K/Akt plays an important role in the regulation of bone-muscle formation and reconstruction.