摘要
为建立有效的α6/α3β2β3 nAChRs(α6/α3β2β3,或简写为α6β2*,*代表其他亚基)体外重组表达实验模型,利用非洲爪蟾卵母细胞表达体系,采用显微注射RNA的方法,利用双电极电压钳技术检测电流,对α6/α3β2β3 nAChRs亚型进行体外重组表达研究,并对其表达条件进行优化,获得了α6/α3β2β3 nAChRs优化的重组表达体系.烟碱型乙酰胆碱受体(nAChRs)是一类重要的配体门控离子通道,其亚型众多且结构相似,但各亚型的生理学和药理学功能截然不同.天然的α6β2*nAChRs很难体外表达,体外表达模型的表达电流非常小.因此,本实验拟研究优化该亚型的表达,进行优化后的体外表达模型,产生的激发电流显著提高,为以该亚型为靶点的新药筛选提供实验模型和基础.
Nicotinic acetylcholine receptors(nAChRs),an important kind of ligand-gated ion channels with many subtypes,are similar in structures but different in physiological and pharmacological functions.Naturalα6β2*nAChRs are unlikely to be expressed in vitro and their expression currents are quite small.In order to establish an effective experimental model ofα6/α3β2β3 nAChRs in vitro,the expression system of Xenopus laevis oocytes was used to express theα6/α3β2β3 nAChRs subtype with the method of micro injection of RNA and detection with two-electrode voltage clamp.The expression conditions were optimized to obtain a better and more efficient expression system forα6/α3β2β3 nAChRs.The current generated by the expressed receptor was significantly increased.This may provide an experimental model and basis for the screening of new drugs targeting this subtype.
作者
施西子
毛楷林
朱晓鹏
长孙东亭
罗素兰
SHI Xizi;MAO Kailin;ZHU Xiaopeng;ZHANGSUN Dongting;LUO Sulan(Key Laboratory of Tropical Biological Resources of Ministry of Education/Key Lab for Marine Drugs of Haikou/School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228,China)
出处
《华南师范大学学报(自然科学版)》
CAS
北大核心
2020年第3期70-77,共8页
Journal of South China Normal University(Natural Science Edition)
基金
国家自然科学基金项目(81872794,41966003)
高等学校学科创新引智计划资助项目(D20010)。