3种不同固定相对皂苷异构体分离度的对比研究

Comparative studies on resolution of saponin isomers by various stationary phases

目的分析3种不同固定相对皂苷异构体的分离效果。方法3对皂苷异构体gypenoside L和gypenoside LI、damulin A和damulin B、20(S)-ginsenoside Rg3和20(R)-ginsenoside Rg3,通过C18色谱柱、五氟苯基丙基色谱柱及双苯基色谱柱进行分离比较。以Inertsill ODS-SP色谱柱、Shim-pack Velox PFPP色谱柱和Shim-pack Velox Bipheny1色谱柱作为固定相,用不同比例的流动相分离gypenoside L、gypenoside LI、damulin A、damulin B、20(S)-ginsenoside Rg3和20(R)-ginsenoside Rg3。结果Shim-pack Velox PFPP色谱柱分别在29%和33%乙腈水溶液以1.0 mL/min等度洗脱,gypenoside L和gypenoside LI的分离度达到2.27,20(S)-ginsenoside Rg3和20(R)-ginsenoside Rg3的分离度达到3.50,分离效果最佳;Shim-pack Velox Biphenyl色谱柱在33%乙腈水溶液以0.5 mL/min等度洗脱,damulinA和damulinB的分离效果最佳,分离度达到2.73。结论五氟苯基丙基色谱柱因键合五氟苯基丙基,对差向异构体具有更好的分离度,双苯基色谱柱因键合二苯基,对位置异构体有更好的分离度。

Objective To analyze the separation effects of 3 different stationary phases on saponin isomer.Methods Three pairs of saponin isomers,gypenoside L and gypenoside LI,damulin A and damulin B,20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3,were compared by C18 column,pentafluorophenylpropyl(PFPP)column and diphenyl column,respectively.Inertsill ODS-SP column,Shim-pack Velox PFPP column and Shim-pack Velox Diphenyl column were used as stationary phases,and gypenoside L,gypenoside LI,damulin A,damulin B,20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3 were separated by different proportions of mobile phase.Results The effects of separation of gypenoside L and gypenoside LI,and 20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3 showed the best using Shim-pack Velox PFPP column.The resolution of gypenoside L and gypenoside LI was 2.27 eluted with 29%acetonitrile and the resolution of 20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3 was 3.50 eluted with 33%acetonitrile at flow rate of 1.0 mL/min;Damulin A and damulin B showed the best separation,with the resolution of 2.73,using Shim-pack Velox Diphenyl column eluted with 33%acetonitrile at the flow rate of0.5 mL/min.Conclusion Pentafluorophenylpropyl column is used to separate the epimers due to the bonding of pentafluorophenylpropyl.Diphenyl column shows better resolution for positional isomers due to the bonding of diphenyl groups.