高效液相色谱-蒸发光散射检测法测量饮料中三氯蔗糖含量的不确定度评估

Uncertainty evaluation for determination of sucralose in beverage by high performance liquid chromatography-evaporative light-scattering detector

目的评定高效液相色谱-蒸发光散射检测法测量饮料中三氯蔗糖含量的不确定度。方法以GB22255-2014《食品中三氯蔗糖的测定》为检测依据,对饮料中的三氯蔗糖含量进行定量分析。以JJF 1059-2012《测量不确定度评定与表示》为依据对测量过程中的不确定度因素进行评估,并计算合成不确定度与扩展不确定度。结果高效液相色谱-蒸发光散射检测法测量饮料中三氯蔗糖含量的不确定度结果表示为(45.0±4.09)mg/kg,k=2(P=95%)。结论饮料中三氯蔗糖含量测定的不确定度主要来源于标准曲线拟合和标准品配制,在实际测量过程中应该注意规范操作,以减少不确定度因素,提高测量结果的准确性。

Objective To evaluate the uncertainty of determination of sucralose in beverage by high performance liquid chromatography-evaporative light-scattering detector(HPLC-ELSD).Methods The content of sucralose in beverage was quantitatively analyzed based on GB 22255-2014 Determination of sucralose in food.The uncertainty of the factors in measurement process was evaluated and the synthetic uncertainty and extension uncertainty were calculated according to JJF 1059-2012 Evaluation and expression of uncertainty in measurement.Results The result of HPLC-ELSD in the determination of sucralose in beverages could be expressed as(45.0±4.09)mg/kg,k=2(P=95%).Conclusion The uncertainty in the determination of sucralose content in beverages mainly comes from the fitting of standard curve and the preparation of standards.In the actual measurement process,the standardized operation is required to reduce the uncertainty factors and improve the accuracy of the measurement results.