基于逆流鞘液的微流控芯片设计及流场分析

Design and Flow Analysis of Microfluidic Chip with Reverse Sheath Fluid

微流控芯片应用于细胞分析分选、微生物检测时,在流动区域中可能会形成驻点,驻点的存在可能会引起流道拥堵、样品污染、纯度下降等问题。针对该问题,设计了一种微流控芯片,利用逆流鞘液避免细胞在驻点区域接触壁面,从而防止细胞聚集阻塞流道,同时对分选通道和废液通道中样品流再次聚焦,使样品流动更加稳定。利用Fluent软件对这种微流控芯片中的流场进行仿真分析,得到其流动状态,验证了引入逆流鞘液对于避免驻点引起的阻塞问题具有一定作用,对设计应用于细胞分析分选的微流控芯片具有参考价值。

The presence of stagnation points may cause problems such as flow channel congestion,sample contamination,and decreasing in purity formed when the microfluidic chip is applied to cell analysis or sorting and microbial detection.Aiming at this problem,a microfluidic chip was designed.The countercurrent sheath liquid was used to prevent the cells from contacting the wall in the stagnation area,thereby to prevent the cells from collecting and blocking the flow channel,and refocus the sample flow in the sorting channel and the waste channel so that the sample flow can be more stable.At the same time,Fluent is used to simulate the flow field in the microfluidic chip to obtain the flow state.It is verified that the introduction of reverse sheath fluid can avoid the problems caused by the stagnation,and it can contribute to the design of microfludic chips for cell analysis and sorting.